Differential sensitivity of gene expression in vitro to inhibitors of DNA gyrase.
Differential sensitivity of gene expression in vitro to inhibitors of DNA gyrase.
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体外基因表达对 DNA 旋转酶抑制剂的敏感性差异。
DOI：
10.1073/pnas.76.7.3304
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发表时间：
1979
影响因子：
11.1
通讯作者：
G. Zubay
中科院分区：
文献类型：
--
作者：
H. Yang;K. Heller;M. Gellert;G. Zubay
我们使用DNA回转酶的特异性抑制剂抗生素库默霉素A1、新生物素和草酸，研究了在DNA导向的无细胞系统中几种细菌和质粒基因的耦合转录和翻译。抑制DNA旋切酶活性可不同程度地降低不同基因的表达。在所检测的基因中，发现colicin E1质粒中的lac操纵子、rRNA基因和colicin基因最敏感，而colicin E1质粒中的trp操纵子和其他一些基因相对不受抑制剂的影响。这些结果，连同早期关于环状DNA模板转录的工作，表明DNA超卷曲可以显著增强某些启动子的转录。
We have used the antibiotics coumermycin A1, novobiocin, and oxolinic acid, which are specific inhibitors of DNA gyrase, to study the coupled transcription and translation of several bacterial and plasmid genes in a DNA-directed cell-free system. The expression of different genes is reduced to different extents by inhibition of DNA gyrase activity. Among the genes tested, the lac operon, an rRNA gene, and the colicin gene of colicin E1 plasmid were found to be most sensitive, while the trp operon and some other genes in colicin E1 plasmid were relatively unaffected by the inhibitors. These results, together with earlier work on the transcription of circular DNA templates, indicate that DNA supercoiling can significantly enhance transcription from certain promoters.