A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni
A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni
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DOI：
10.1590/s0074-02762006000900027
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发表时间：
2006-10-01
期刊：
影响因子：
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通讯作者：
Loker, Eric S
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文献类型：
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作者：
Adema, Coen M;Luo, Mei-Zhong;Loker, Eric S
为了提供一种新的资源用于分析光滑双脐菌的基因组，国际光滑双脐菌基因组计划（biology.umn.edu/biomphalaria-genome.html）的成员与亚利桑那基因组研究所（AGI）合作，并得到国家人类基因组研究所（NHGRI）的支持，产生了一个高质量的细菌人工染色体（BAC）文库。BB 0 2菌株B. glabrata是一种对曼氏血吸虫的几个品系敏感的田间分离株（Belo Horizonte，米纳斯吉拉斯，巴西），将其自交两代以减少后代中的单倍型多样性。从40只蜗牛的卵睾丸中提取高分子量DNA，用HindIII部分消化，并连接到pAGIBAC 1载体中，得到B。glabrata BAC文库（BG_BBa）由61824个克隆（136.3kb平均插入片段大小）组成，并提供9.05x的931 Mb基因组覆盖率。用来自B的单/低拷贝数基因进行探测。glabrata和所选BAC克隆的指纹分析表明BAC文库充分代表了基因互补。BAC末端序列（514 reads，299860 nt）表明，B. glabrata含有63%的AT，并揭示了几个新的基因，转座因子和高频序列元件组。该BG_BBa BAC文库可从AGI以研究团体的成本获得，由于BB 02菌株B. glabrata是通过NHGRI进行的靶向全基因组测序。
To provide a novel resource for analysis of the genome of Biomphalaria glabrata, members of the international Biomphalaria glabrata Genome Initiative (biology.umn.edu/biomphalaria-genome.html), working with the Arizona Genomics Institute (AGI) and supported by the National Human Genome Research Institute (NHGRI), produced a high quality bacterial artificial chromosome (BAC) library. The BB02 strain B. glabrata, a field isolate (Belo Horizonte, Minas Gerais, Brasil) that is susceptible to several strains of Schistosoma mansoni, was selfed for two generations to reduce haplotype diversity in the offspring. High molecular weight DNA was isolated from ovotestes of 40 snails, partially digested with HindIII, and ligated into pAGIBACl vector The resulting B. glabrata BAC library (BG_BBa) consists of 61824 clones (136.3 kb average insert size) and provides 9.05 x coverage of the 931 Mb genome. Probing with single/low copy number genes from B. glabrata and fingerprinting of selected BAC clones indicated that the BAC library sufficiently represents the gene complement. BAC end sequence data (514 reads, 299860 nt) indicated that the genome of B. glabrata contains similar to 63% AT and disclosed several novel genes, transposable elements, and groups of high frequency sequence elements. This BG_BBa BAC library, available from AGI at cost to the research community, gains in relevance because BB02 strain B. glabrata is targeted whole genome sequencing by NHGRI.