A GENETIC-MAP OF POTATO (SOLANUM-TUBEROSUM) INTEGRATING MOLECULAR MARKERS, INCLUDING TRANSPOSONS, AND CLASSICAL MARKERS
A GENETIC-MAP OF POTATO (SOLANUM-TUBEROSUM) INTEGRATING MOLECULAR MARKERS, INCLUDING TRANSPOSONS, AND CLASSICAL MARKERS
复制标题
DOI：
10.1007/bf00220891
复制
发表时间：
1995-07-01
影响因子：
5.4
通讯作者：
STIEKEMA, WJ
中科院分区：
文献类型：
--
作者：
JACOBS, JME;VANECK, HJ;STIEKEMA, WJ
利用 67 个二倍体马铃薯植株的回交群体构建了马铃薯 (Solanum tuberosum L.) 的遗传图谱，该图谱整合了分子标记与形态学和同工酶标记。描述了图谱构建的一般方法，该方法不同于以前在马铃薯和其他近交植物中使用的方法。首先，为女性和男性父母构建了单独的地图。女性地图包含 132 个标记，而男性地图包含 138 个标记。其次，根据共同标记，通过计算机程序 JoinMap 将两个集成的亲本图合并为一个。该组合图谱由 175 个分子标记、10 个形态标记和 8 个同工酶标记组成。其中 92 个分子标记源自马铃薯 T-DNA 插入片段或源自这些 T-DNA 构建体的重新整合的玉米转座元件侧翼的 DNA 序列。在父本的 1、2、8 和 11 号染色体以及双亲的 5 号染色体上发现了扭曲的分离簇。组合地图的总长度为1120厘米。
A genetic map of potato (Solanum tuberosum L.) integrating molecular markers with morphological and isozyme markers was constructed using a backcross population of 67 diploid potato plants. A general method for map construction is described that differs from previous methods employed in potato and other outbreeding plants. First, separate maps for the female and male parents were constructed. The female map contained 132 markers, whereas the male map contained 138 markers. Second, on the basis of the markers in common the two integrated parental maps were combined into one with the computer programme JoinMap. This combined map consisted of 175 molecular markers, 10 morphological markers and 8 isozyme markers. Ninety-two of the molecular markers were derived from DNA sequences flanking either T-DNA inserts in potato or reintegrated maize transposable elements originating from these T-DNA constructs. Clusters of distorted segregation were found on chromosomes 1, 2, 8 and 11 for the male parent and chromosome 5 for both parents. The total length of the combined map is 1120 cM.