Novel detoxification of the trichothecene mycotoxin deoxynivalenol by a soil bacterium isolated by enrichment culture
Novel detoxification of the trichothecene mycotoxin deoxynivalenol by a soil bacterium isolated by enrichment culture
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DOI：
10.1128/aem.63.10.3825-3830.1997
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发表时间：
1997-10-01
影响因子：
4.4
通讯作者：
Ochi, K
中科院分区：
文献类型：
--
作者：
Shima, J;Takase, S;Ochi, K
通过富集培养，从土壤样品中获得了一株能代谢脱氧雪腐镰刀菌烯醇的混合微生物。从富集培养物中分离到一株细菌（E3-39），培养1天后可完全去除培养基中外源提供的脱氧雪腐镰刀菌烯醇。根据形态学、生理学和系统发育研究，将菌株E3-39分类为土壤杆菌-根瘤菌群。薄层色谱分析表明，E3-39菌株乙酸乙酯提取物中存在一种主要的脱氧雪腐镰刀菌烯醇代谢产物和两种次要的代谢产物，经质谱、H-1和C-13核磁共振分析，确定主要代谢产物为3-酮基-4-脱氧雪腐镰刀菌烯醇。通过基于有丝分裂原诱导的和无丝分裂原的小鼠脾淋巴细胞增殖的生物测定来评价3-酮-4-脱氧雪腐镰刀菌烯醇的免疫抑制毒性，与脱氧雪腐镰刀菌烯醇相比，该化合物的免疫抑制毒性显著降低（低于十分之一），表明脱氧雪腐镰刀菌烯醇中的3-OH基团可能参与发挥其免疫抑制毒性。菌株E3-39也能够转化3-乙酰基脱氧雪腐镰刀菌烯醇，但不能转化雪腐镰刀菌烯醇和镰孢菌酮-X。
A mixed microbial culture capable of metabolizing deoxynivalenol was obtained from soil samples by an enrichment culture procedure. A bacterium (strain E3-39) isolated from the enrichment culture completely removed exogenously supplied deoxynivalenol from culture medium after incubation for 1 day, On the basis of morphological, physiological, and phylogenetic studies, strain E3-39 was classified as a bacterium belonging to the Agrobacterium-Rhizobium group. Thin-layer chromatographic analysis indicated the presence of one major and two minor metabolites of deoxynivalenol in ethyl acetate extracts of the E3-39 culture filtrates, The main metabolite was identified as 3-keto-4-deoxynivalenol by mass spectroscopy and H-1 and C-13 nuclear magnetic resonance analysis, The immunosuppressive toxicity of 3-keto-4-deoxynivalenol was evaluated by means of a bioassay based on the mitogen-induced and mitogen-free proliferations of mouse spleen lymphocytes, This compound exhibited a remarkably decreased (to less than one tenth) immunosuppressive toxicity relative to deoxynivalenol, indicating that the 3-OH group in deoxynivalenol is likely to be involved in exerting its immunosuppressive toxicity, Strain E3-39 was also capable of transforming 3-acetyldeoxynivalenol but not nivalenol and fusarenon-X.