Improving osteogenesis of calcium phosphate bone cement by incorporating with lysine: An in vitro study
Improving osteogenesis of calcium phosphate bone cement by incorporating with lysine: An in vitro study
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通过加入赖氨酸改善磷酸钙骨水泥的成骨作用：一项体外研究
DOI：
10.1016/j.colsurfb.2019.02.034
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发表时间：
2019-05-01
影响因子：
5.8
通讯作者：
Ye, Jiandong
中科院分区：
文献类型：
--
作者：
Shi, Haishan;Ye, Xiaoling;Ye, Jiandong
磷酸钙骨水泥（CPC）引起了外科医生和材料科学家的广泛兴趣。但由于其成骨能力差，限制了其实际应用。本研究将蛋白质的重要成分赖氨酸引入CPC中，以提高CPC的成骨能力。研究了赖氨酸对CPC的物相、形态、理化性质、蛋白质吸附、赖氨酸释放及细胞相容性的影响。结果表明，赖氨酸对水化水泥的物相和形貌无明显影响，但能显著提高水泥的抗压强度、显气孔率和凝结时间，且随赖氨酸含量的增加而增加。与对照组相比，赖氨酸掺入的CPC具有显著增强的体外成骨能力。这可能与骨水泥增强了纤维连接蛋白（FN）的锚定和骨髓间充质干细胞（BMSCs）的粘附以及生物活性氨基酸分子的持续释放有关。因此，赖氨酸有望作为一种新型的生物活性混合物在CPC的开发与改善成骨能力和许多骨科应用的理化性质。
Calcium phosphate bone cement (CPC) has attracted extensive interests from surgeons and material scientists. However, its actual application is still limited because of its poor osteogenesis. In this work, lysine, one of the essential components of proteins, was incorporated into the CPC to improve its osteogenesis ability. Effects of lysine on the phase, morphology, physicochemical properties, protein adsorption, lysine release and cyto-compatibility of CPC were investigated. Results showed that lysine had no significant influence on the phase and morphology of the hydrated cements, but evidently raised the compressive strength, apparent porosity and setting time of the cements in a content-dependent manner of lysine. In contrast to the control, the lysine incorporated CPCs had notably enhanced in vitro osteogenesis capability. It was supposed to be synergistically attributed to the improvements of fibronectin (FN) anchoring and bone mesenchymal stem cells (BMSCs) adhesion on the hydrated cements as well as the sustained release of bioactive amino acid molecules. Hence, lysine was expected to be applied as a novel bioactive admixture in the development of CPC with the improved osteogenesis ability and physicochemical properties for numerous orthopedic applications.