All-digital image capture and whole-field analysis of ciliary beat frequency
All-digital image capture and whole-field analysis of ciliary beat frequency
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DOI：
10.1046/j.1365-2818.2003.01209.x
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发表时间：
2003-08-01
期刊：
影响因子：
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通讯作者：
Wyatt, TA
中科院分区：
文献类型：
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作者：
Sisson, JH;Stoner, JA;Wyatt, TA
我们假设，一个高速的全数字视频成像系统，与计算机化的分析，将精确地捕获和测量纤毛搏动频率（CBF），并缩短从数据捕获到数据分析的时间。我们比较了一个传统的模拟视频系统与一个新的高速数字系统，我们开发的CBF分析。使用纤毛原代牛支气管上皮细胞，我们同时模拟和数字CBF测量的同一区域的利益（ROI），而温度是不同的。这产生了几乎相同的数据在很宽的频率范围内（7-15赫兹）使用任何一个系统。然而，与数字系统不同的是，模拟系统不能准确地检测15 Hz以上的CBF（温度高于30 ℃）。我们还比较了ROI分析与一种新的分析算法，我们命名为全场分析（WFA）。与模拟系统相比，CBF的WFA测量与ROI一致，并将分析数据所需的操作时间减少了90%以上。我们得出的结论是，全数字计算机化CBF分析与标准视频方法密切相关，显着加快了数据分析速度，并提供了新的方法（包括WFA）来同时分析整个活动纤毛区域。我们称这个系统为“Sisson-Ammons视频分析”（SAVA）。
We hypothesized that a high-speed all-digital video imaging system, with computerized analysis, would precisely capture and measure ciliary beat frequency (CBF) and would shorten the time from data capture to data analysis. We compared a conventional analog video system with a new high-speed digital system we developed for CBF analysis. Using ciliated primary bovine bronchial epithelial cells we made simultaneous analog and digital CBF measurements of the same region of interest (ROI) while temperature was varied. This yielded nearly identical data over a wide range of frequencies (7-15 Hz) using either system. Unlike the digital system however, the analog system did not accurately detect CBF above 15 Hz (temperatures higher than 30 degreesC). We also compared ROI analysis with a new analysis algorithm we have named whole-field analysis (WFA). WFA measurement of CBF agreed with ROI and reduced operator time required to analyse data by more than 90% compared with the analog system. We conclude that all-digital computerized CBF analysis correlates closely with standard video methods, markedly speeds up data analysis and provides new ways, including WFA, to analyse entire fields of motile cilia simultaneously. We have termed this system 'Sisson-Ammons Video Analysis' (SAVA).