Behavior and mutagenesis screens: the importance of baseline analysis of inbred strains
Behavior and mutagenesis screens: the importance of baseline analysis of inbred strains
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DOI：
10.1007/s003350010107
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发表时间：
2000-07-01
期刊：
影响因子：
2.5
通讯作者：
Bucan, M
中科院分区：
文献类型：
--
作者：
Tarantino, LM;Gould, TJ;Bucan, M
随机突变作为鉴定基因功能的一种手段，已广泛应用于各种模式生物。直到最近，它一直主要用于识别导致可见和发育突变的单基因性状。然而，这种遗传学方法也有能力识别控制复杂生物系统（如行为）的基因。行为突变的诱变筛选需要仔细考虑许多因素，包括选择用于诱变的测定和背景菌株以及随后对受影响的一个或多个基因进行作图。本文介绍了行为检测监测运动协调加速旋转棒，焦虑相关的行为，高架零迷宫和感觉运动反应，门控，习惯性的声惊吓。这五种生理或神经行为可以代表各种神经和精神疾病的潜在内表型。在这些行为测定中，四种近交系小鼠（C57 BL/6 J、C3 HeB/FeJ、DBA/2 J和129/SvlmJ）的表现存在显著的品系特异性和性别特异性差异，这说明了在进行行为诱变筛选和选定突变的遗传作图之前进行基线分析的重要性。
Random mutagenesis as a means of identifying the function of genes has been used extensively in a variety of model organisms. Until recently it has been used primarily in the identification of single-gene traits that cause visible and developmental mutations. However, this genetic approach also has the power to identify genes that control complex biological systems such as behavior. Mutagenesis screens for behavioral mutations require careful consideration of many factors, including choice of both assays and background strains for use in mutagenesis and subsequent mapping of the affected gene or genes. This paper describes behavioral assays for monitoring motor coordination on the accelerating rotarod, anxiety-related behaviors in the elevated zero maze and sensorimotor reactivity, gating, and habituation of acoustic startle. These five physiological or neurological behaviors can represent potential endophenotypes for a variety of neurological and psychiatric disorders. The significant degree of strain- and sex-specific differences in the performance of four inbred strains of mice (C57BL/6J, C3HeB/FeJ, DBA/2J, and 129/SvlmJ) in these behavioral assays illustrates the importance of performing baseline analysis prior to behavioral mutagenesis screens and genetic mapping of selected mutations.