Molecular genetic maps of the group 6 chromosomes of hexaploid wheat (Triticum aestivum L em Thell)
Molecular genetic maps of the group 6 chromosomes of hexaploid wheat (Triticum aestivum L em Thell)
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作者：
Marino, CL;Nelson, JC;Hart, GE
六倍体小麦 (Triticum aestivum L. em. Thell.) 6A、6B 和 6D 染色体的限制性片段长度多态性 (RFLP) 图谱已经生成。它们是使用源自合成小麦与面包小麦杂交的 F-7-8 重组自交系群体构建的。这些图谱包含 74 个分配给 LOD 大于或等于 3 的图谱位置的标记（29 个标记分配给 6A、24 至 6B 和 21 至 6D），以及 2 个分配给 6D 的标记（以 LOD 为 2.7 排序）。另外 78 个标记被分配到地图上的区间。 6A、6B 和 6D 的图跨度分别为 178、132 和 206 cM。 21 个克隆在两个同源物中检测到直系同源基因座，3 个克隆在每条染色体中检测到直系同源基因座。直系同源基因座以1.5至26cM的间隔位于连锁图谱70%的长度上。在这部分图谱中，强烈表明了三个同源物之间的共线性（同源性）。连锁图的其余部分由三个长度范围为 47 至 60 cM 的片段组成。指出了这些染色体与其他小麦科同源 6 组染色体之间的共线性，并提出了从六倍体小麦、四倍体小麦、小麦和大麦同源 6 组染色体图谱衍生的共有 RFLP 图谱。
Restriction fragment length polymorphism (RFLP) maps of chromosomes 6A, 6B, and 6D of hexaploid wheat (Triticum aestivum L. em. Thell.) have been produced. They were constructed using a population of F-7-8 recombinant inbred lines derived from a synthetic wheat x bread wheat cross. The maps consist of 74 markers assigned to map positions at a LOD greater than or equal to 3 (29 markers assigned to 6A, 24 to 6B, and 21 to 6D) and 2 markers assigned to 6D ordered at a LOD of 2.7. Another 78 markers were assigned to intervals on the maps. The maps of 6A, 6B, and 6D span 178, 132, and 206 cM, respectively. Twenty-one clones detected orthologous loci in two homoeologues and 3 detected an orthologous locus in each chromosome. Orthologous loci are located at intervals of from 1.5 to 26 cM throughout 70% of the length of the linkage maps. Within this portion of the maps, colinearity (homosequentiality) among the three homoeologues is strongly indicated. The remainder of the linkage maps consists of three segments ranging in length from 47 to 60 cM. Colinearity among these chromosomes and other Triticeae homoeologous group 6 chromosomes is indicated and a consensus RFLP map derived from maps of the homoeologous group 6 chromosomes of hexaploid wheat, tetraploid wheat, Triticum tauschii, and barley is presented.