Neuroprotective effect of Eleutheroside B on 1-methyl-4-phenylpyridinium ion-induced apoptosis in PC12 cells
Neuroprotective effect of Eleutheroside B on 1-methyl-4-phenylpyridinium ion-induced apoptosis in PC12 cells
复制标题
刺五加苷 B 对 1-甲基-4-苯基吡啶鎓离子诱导的 PC12 细胞凋亡的神经保护作用
DOI：
10.3969/j.issn.1673-5374.2011.18.003
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发表时间：
2011-06-01
影响因子：
6.1
通讯作者：
Tang, Bo
中科院分区：
文献类型：
--
作者：
Lu, Fang;Dong, Yang;Tang, Bo
采用流式细胞术、Annexin V-碘化丙啶双标记和3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四氮唑溴化物检测1-甲基-4-苯基吡啶离子（MPP+）诱导的PC 12细胞损伤后细胞凋亡和存活率。采用紫外分光光度法测定乳酸脱氢酶、超氧化物歧化酶活性和丙二醛含量。流式细胞仪检测线粒体膜电位和细胞内钙离子浓度的变化，Western blot检测caspase-3活性。根据细胞活力和凋亡研究，MPP+诱导的PC 12细胞凋亡在10 μ g/mL的刺五加苷B的存在下被抑制。结果表明，刺五加苷B对MPP+诱导的PC 12细胞凋亡具有神经保护作用，其机制可能与提高细胞抗氧化应激能力、维持线粒体膜电位的高能状态、降低细胞内钙离子浓度和抑制caspase-3活性有关。
Apoptosis and viability of PC12 cells following 1-methyl-4-phenylpyridinium ion (MPP+)-induced injury were monitored by flow cytometry, following Annexin V-propidium iodide double labeling, and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, respectively. The release of lactate dehydrogenase, superoxide dismutase activity and levels of malondialdehyde were determined by UV spectrophotometry. The changes in mitochondrial membrane potential and the intracellular concentration of calcium were determined by flow cytometry, and the activity of caspase-3 was monitored by western blot. According to cell viability and apoptosis studies, MPP+-induced apoptosis in PC12 cells was inhibited in the presence of 10 mu g/mL of Eleutheroside B. Our results indicate that the neuroprotective effect of Eleutheroside B, following MPP+-induced apoptosis in PC12 cells, involves increasing the anti-oxidative stress capacity of cells, maintaining the high-energy state of mitochondrial membrane potential, reducing intracellular calcium concentration and inhibiting caspase-3 activity.