A novel Toll like receptor with two TIR domains (HcToll-2) is involved in Cross Mark regulation of antimicrobial peptide gene expression of Hyriopsis cumingii
A novel Toll like receptor with two TIR domains (HcToll-2) is involved in Cross Mark regulation of antimicrobial peptide gene expression of Hyriopsis cumingii
复制标题
具有两个 TIR 结构域的新型 Toll 样受体 (HcToll-2) 参与三角帆蚌抗菌肽基因表达的交叉标记调控
DOI：
10.1016/j.dci.2014.02.020
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发表时间：
2014-07-01
影响因子：
2.9
通讯作者：
Wang, Jin-Xing
中科院分区：
文献类型：
--
作者：
Ren, Qian;Lan, Jiang-Feng;Wang, Jin-Xing
动物Toll样受体(TLRs)参与先天免疫。Toll蛋白通常是跨膜蛋白。在本研究中，从三角贝中发现了一种非典型的Toll样受体(HcToll-2)。与典型的具有细胞外富含亮氨酸重复序列(LRR)、跨膜和细胞内Toll/白细胞介素1受体(TIR)结构域的Toll样受体不同，HcToll-2在C末端有两个同源的TIR结构域(命名为HcTIR1和HcTIR2)，并且没有跨膜结构域。系统进化分析表明，HcTIR1与海葵Toll的TIR聚为一类，HcTIR2与果蝇Toll的TIR聚为一类。HcToll-2mRNA在肝胰腺中可检测到，经大肠杆菌和金黄色葡萄球菌攻击后表达上调。带有GST标签的重组HcLRR蛋白可与细菌结合，也可与内毒素和PGN结合。HcTIR1和HcTIR2基因在果蝇S2细胞中的过表达RNAi分析表明，HcToll-2是富含半胱氨酸的抗菌肽(AMP)基因，它的表达需要HcToll-2。本研究首次报道了一种非典型的Toll样受体HcToll-2通过诱导AMP的表达参与抗菌免疫。(C)2014爱思唯尔有限公司。保留所有权利。
Animal Toll-like receptors (TLRs) are involved in innate immunity. Toll proteins are generally transmembrane proteins. In this study, an atypical Toll-like receptor (HcToll-2) was identified from the triangle-shell pearl mussel Hyriopsis cumingii, which belongs to phylum Mollusca. Unlike the typical Toll like receptors with extracellular leucine-rich repeats (LRRs), transmembrane, and intracellular Toll/interleukin-1 receptor (TIR) domains, HcToll-2 has two homologous TIR domains located at the C-terminal (designated as HcTIR1 and HcTIR2) and lacks a transmembrane domain. Phylogenetic analysis showed that HcTIR1 was clustered with TIR of sea anemone Toll, and HcTIR2 was clustered with TIR of Drosophila Toll. HcToll-2 mRNA could be detected in the hepatopancreas and was upregulated after challenge with Escherichia coli and Staphylococcus aureus. Recombinant HcLRR protein with GST tag could bind to bacteria and also to LPS and PGN. Over-expression of both HcTIR1 and HcTIR2 induced drosomycin genes in Drosophila S2 cells. RNAi analysis showed that HcToll-2 was required for the expression of theromacin, which is a cysteine-rich antimicrobial peptide (AMP) gene. This research is the first report of an atypical Toll-like receptor HcToll-2 involved in antibacterial immunity through induction of AMP expression. (C) 2014 Elsevier Ltd. All rights reserved.