Human TAF(parallel to)105 is a cell type-specific TFIID subunit related to hTAF(parallel to)130
Human TAF(parallel to)105 is a cell type-specific TFIID subunit related to hTAF(parallel to)130
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DOI：
10.1016/s0092-8674(00)81330-6
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发表时间：
1996-10-04
期刊：
影响因子：
64.5
通讯作者：
Tjian, R
中科院分区：
文献类型：
--
作者：
Dikstein, R;Zhou, S;Tjian, R
我们之前对果蝇和人类 TAF 亚基进行了表征，这些亚基构成了所有细胞中发现的核心 TFIID 复合物。在此，我们报告分化的 B 细胞含有一种新型的 105 kDa 亚化学计量 TAF，未发现与从其他细胞类型中分离出的 TFIID 相关。编码 hTAF(II)105 的 cDNA 揭示了 hTAF(II)130 和 dTAF(II)110 共有的高度保守的 C 端结构域，而 N 端共激活结构域却存在显着差异。所有测试的细胞均表达 TAF(II)105 mRNA，但只有 B 细胞含有显着水平的与 TFIID 相关的蛋白质。 hTAF(II)105 的瞬时过表达选择性抑制 B 细胞中某些基因的转录。这些特性表明 TAF(II)105 是 TFIID 的细胞类型特异性亚基，可能负责介导 B 细胞中激活剂子集的转录。
We previously characterized Drosophila and human TAF subunits that make up the core TFIID complex found in all cells. Here, we report that differentiated B cells contain a novel substoichiometric TAF of 105 kDa not found associated with TFIID isolated from other cell types. The cDNA encoding hTAF(II)105 reveals a highly conserved C-terminal domain shared by hTAF(II)130 and dTAF(II)110, while the N-terminal coactivator domain has diverged significantly. All cells tested express TAF(II)105 mRNA, but only B cells contain significant levels of protein associated with TFIID. Transient overexpression of hTAF(II)105 selectively squelches the transcription of some genes in B cells. These properties suggest that TAF(II)105 is a cell type-specific subunit of TFIID that may be responsible for mediating transcription by a subset of activators in B cells.