Perineuronal germinal cells in the rat cerebral cortex
Perineuronal germinal cells in the rat cerebral cortex
复制标题
DOI：
10.1007/s00795-006-0307-x
复制
发表时间：
2006-03-01
影响因子：
1.8
通讯作者：
Yamada, Hisao
中科院分区：
文献类型：
--
作者：
Kataoka, Yosky;Tamura, Yasuhisa;Yamada, Hisao
成年哺乳动物中枢神经系统中细胞的产生和更新维持大脑功能，包括可塑性。即使在成年哺乳动物的大脑皮层中，神经胶质细胞也被认为每100天就会被新产生的细胞所取代。最近，我们证明了这种增殖是由神经活动刺激的。然而，在皮层实质中是否存在任何germinal区域是未知的。在这里，我们研究了在成年大鼠大脑皮质的增殖细胞动力学，使用BrdU标记和免疫组化NeuN和核纤层蛋白B1。单次注射BrdU后2h，在神经元周围区域观察到超过80%的BrdU标记细胞，其中BrdU标记细胞核位于神经元核的5 μ m内。注射BrdU后2周内，BrdU标记细胞中神经束膜细胞与非神经束膜细胞的比例逐渐下降。这些观察结果表明，许多皮质细胞增殖的神经元周围的领土，germinant土壤，这些新产生的细胞的一部分迁移到周围地区的神经元周围的领土在2周后的有丝分裂。
The generation and renewal of cells in the adult mammalian central nervous system maintains brain functions, including plasticity. Even in the cerebral cortex of adult mammals, glial cells are thought to be replaced with newly generated cells every 100 days. Recently, we demonstrated that this proliferation is stimulated by neural activity. However, whether any germinal areas exist in the cortical parenchyma is unknown. Here, we examined the proliferating cell dynamics in the cerebral cortex of adult rats using BrdU labeling and immunohistochemistry for NeuN and lamin B1. At 2h after a single injection of BrdU, more than 80% of BrdU-labeled cells were observed in the perineuronal territory in which the BrdU-labeled nuclei were located within 5 mu m from neuronal nuclei. The ratio of perineuronal cells to nonperineuronal cells in BrdU-labeled cells gradually decreased over the 2 weeks following BrdU injection. These observations indicate that numerous cortical cells proliferate in the perineuronal territory, the germinal soil, and that part of these newly generated cells migrate from the perineuronal territory into the surrounding areas during the 2 weeks following mitosis.