Self-assembled peptide nanoparticles as tumor microenvironment activatable probes for tumor targeting and imaging.
Self-assembled peptide nanoparticles as tumor microenvironment activatable probes for tumor targeting and imaging.
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DOI：
10.1016/j.jconrel.2013.12.037
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发表时间：
2014-03
期刊：
影响因子：
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通讯作者：
Ying Zhao;T. Ji;Hai Wang;Suping Li;Yuliang Zhao;Guangjun Nie
中科院分区：
文献类型：
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作者：
Ying Zhao;T. Ji;Hai Wang;Suping Li;Yuliang Zhao;Guangjun Nie
针对肿瘤微环境设计特异、灵敏的成像探针，有望实现对肿瘤组织的精确检测和快速反应。pH失调是肿瘤微环境最显著的特征之一，可以被认为是设计广谱和局部环境响应成像探针的良好特异性触发因素。然而，目前现有的pH响应系统的设计策略不足以满足快速和肿瘤特异性诊断的需求。本文报道了一种新型的基于寡肽自组装的仿生纳米结构，当肿瘤微环境中pH值发生微小变化（pH6.8vs.7.4）时，该纳米结构可以快速地从荧光猝灭的自组装阶段切换到荧光活性的解离阶段。这种寡肽组装体作为肿瘤微环境可激活探针进行检查，用于肿瘤内和静脉内体内肿瘤成像。通过不同的荧光强度，验证了酸性肿瘤微环境可以激活更强的荧光信号。量身定制的自组装寡肽纳米材料具有高效、特异性地对各种具有弱酸性微环境的实体肿瘤进行原位诊断的潜力，预计对临床肿瘤诊断至关重要。
Design of specific and sensitive imaging probes for targeting tumor microenvironment holds great promise to achieve precise detection and rapid responsiveness to neoplastic tissues. Dysregulated pH, one of the most remarkable hallmarks of tumor microenvironment, can be considered as a good specific trigger for the design of broad-spectrum and local-environment responsive imaging probes. However, the current existing design strategies for pH-responsive systems are insufficient to meet the needs for a rapid and tumor-specific diagnosis. Here we reported a novel biomimetic nanostructure based on oligopeptide self-assembly that can quickly switch into dissociated stage with active fluorescence property from self-assembled stage with quenched fluorescence activity when encountering a subtle pH-change in tumor microenvironment (pH 6.8vs.7.4). This oligopeptide-assembly is examined as tumor microenvironment activatable probes for both intratumoral and intravenousin vivotumor imaging. Through the distinct fluorescent intensities, it is validated that the acidic tumor microenvironment can activate stronger fluorescence signals. The tailor-made self-assembled oligopeptide nanomaterials have the potential for efficient and specificin situdiagnosis of various solid tumors with a weakly acidic microenvironment, which is expected to be of crucial importance for clinical tumor diagnostics.