Plaunotol induces apoptosis of gastric cancer cells
Plaunotol induces apoptosis of gastric cancer cells
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DOI：
10.1055/s-2007-981578
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发表时间：
2007-08-01
期刊：
影响因子：
2.7
通讯作者：
Nagawa, Hirokazu
中科院分区：
文献类型：
--
作者：
Yamada, Jun;Kawai, Kazushige;Nagawa, Hirokazu
虽然一些异戊二烯类化合物，如紫杉醇和香叶醇(GGOH)已被报道具有很强的抗癌活性，但从白花蛇舌叶中提取的异戊二烯醇(Plaunotol)对癌症的作用尚未被评估。在这里，我们的目的是研究Plaunotol对胃癌细胞株的作用。选用MKN-45、MKN-74和AZ-521三种胃癌细胞株。在10、20、30和40mU/L的浓度下，普鲁托醇呈剂量依赖性地抑制所有胃癌细胞的生长，并依赖于诱导细胞凋亡。在凋亡细胞中发现caspase-8、-9和-3被激活。Bax蛋白表达增加，但对Bcl2和Bclxl蛋白表达无明显影响。普罗托尔应该是一种很有前途的新的抗癌药物，由于它已经在日本投入临床使用，其抗癌特性应该在临床试验中得到证实。
Although some isoprenoids, such as taxans and geranylgeraniol (GGOH), have been reported to have strong anticancer activities, the effect of plaunotol, the isoprenoid extracted from the leaves of Plau-noi, on cancer has not yet been evaluated. Here, we aimed to investigate the effect of plaunotol on gastric cancer cell lines. Three gastric cancer cell lines, namely MKN-45, MKN-74 and AZ-521 were used. Plaunotol was tested at 10, 20, 30 and 40 mu mol/L. Plaunotol dose-dependently inhibited the growth of all gastric cancer cells, dependent on the induction of apoptosis. Caspases-8, -9 and -3, were found to be activated in the apoptotic cells. The expression of Bax protein was increased, but Bcl-2 and Bcl-xL protein expressions were not significantly affected. Plaunotol should be a promising new antitumor agent, and since it is already available for clinical use in Japan, its anticancer properties should be confirmed in clinical trials.