TRANSCRIPTIONAL REGULATION OF THE REDD TRANSCRIPTIONAL ACTIVATOR GENE ACCOUNTS FOR GROWTH-PHASE-DEPENDENT PRODUCTION OF THE ANTIBIOTIC UNDECYLPRODIGIOSIN IN STREPTOMYCES COELICOLOR A3(2)
TRANSCRIPTIONAL REGULATION OF THE REDD TRANSCRIPTIONAL ACTIVATOR GENE ACCOUNTS FOR GROWTH-PHASE-DEPENDENT PRODUCTION OF THE ANTIBIOTIC UNDECYLPRODIGIOSIN IN STREPTOMYCES COELICOLOR A3(2)
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DOI：
10.1111/j.1365-2958.1992.tb01459.x
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发表时间：
1992-10-01
影响因子：
3.6
通讯作者：
BIBB, MJ
中科院分区：
文献类型：
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作者：
TAKANO, E;GRAMAJO, HC;BIBB, MJ
redD是天蓝色链霉菌A3（2）生产红色抗生素十一烷基灵菌红素所需的激活基因，其转录在从指数期到稳定期的过渡期间显示出显著增加。redD表达的增加之后是redX（一种生物合成结构基因）的转录和菌丝体中抗生素的出现，并且与ppGpp的细胞内出现一致。然而，ppGpp生产引起的营养下移，或添加丝氨酸异羟肟酸，指数增长的文化没有刺激作用redD转录。redD在多拷贝质粒上的存在导致redD转录物水平升高，并在指数生长期间产生redX和十一烷基灵菌红素;十一烷基灵菌红素的正常生长阶段依赖性生产似乎完全通过redD启动子介导，其与主要类别的真细菌启动子的共有序列相似性有限。
Transcription of redD, the activator gene required for production of the red-pigmented antibiotic undecylprodigiosin by Streptomyces coelicolor A3(2), showed a dramatic increase during the transition from exponential to stationary phase. The increase in redD expression was followed by transcription of redX, a biosynthetic structural gene, and the appearance of the antibiotic in the mycelium, and coincided with the intracellular appearance of ppGpp. However, ppGpp production elicited either by nutritional shift-down of, or addition of serine hydroxamate to, exponentially growing cultures had no stimulatory effect on redD transcription. The presence of redD on a multicopy plasmid resulted in elevated levels of the redD transcript and production of redX and undecylprodigiosin during exponential growth; the normal growth-phase-dependent production of undecylprodigiosin appeared to be mediated entirely through the redD promoter, which shows limited similarity to the consensus sequence for the major class of eubacterial promoters.