The evolution of Epstein-Barr virus inferred from the conservation and mutation of the virus glycoprotein gp350/220 gene
The evolution of Epstein-Barr virus inferred from the conservation and mutation of the virus glycoprotein gp350/220 gene
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DOI：
10.1007/s11262-008-0323-0
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发表时间：
2009-04-01
期刊：
影响因子：
1.6
通讯作者：
Inoue, Yoshitsugu
中科院分区：
文献类型：
--
作者：
Kawaguchi, Asako;Kanai, Kyosuke;Inoue, Yoshitsugu
为了研究EB病毒（EBV）的变异，我们分析了gp 350/220基因的几个细胞系和日本野生株使用直接测序。除Jijoye/P3 HR-1和少数分离株外，所有EBV的N-末端区域高度保守。该区域的变异与EBV A型和B型（也称为1型和2型）一致，分别称为a型和B型。A/a型在大多数日本细胞系和野生型分离株中检测到，并被归类为具有潜伏膜蛋白（LMP）1基因的China 1型。仅在少数具有Med和China 2型的野生分离物中检测到B/B型。C-末端的多样性高于N-末端，缺乏A/a型和B/B型之间的差异。gp 350/220和LMP 1基因的系统发育分析提示EBV可能存在A/a型和B/B型再向LMP 1亚型进化的模式。
To study variations of Epstein-Barr virus (EBV), we analyzed the gp350/220 gene for several cell lines and Japanese wild isolates using direct sequencing. The N-terminal region was highly conserved in all EBVs except for Jijoye/P3HR-1 and a few isolates. The variation of the region coincided with EBV types A and B (also referred to as types 1 and 2) and were, respectively, designated as the types a and b. The type A/a was detected in most Japanese cell lines and wild isolates, and was classified as China1 type with latent membrane protein (LMP) 1 gene. The type B/b was detected in only a few wild isolates with the Med and China2 types. The C-terminus had more diversity than the N-terminus and lacked the divergence between types A/a and B/b. The phylogenetic analyses of the gp350/220 and LMP1 genes may suggest a mode of EBV evolution into types A/a and B/b and then to LMP1 subtypes.