9,10-(Divinyl) Anthracene Based Bright Aggregation-Induced Emission Organic Dots for Hela Cells Imaging
9,10-(Divinyl) Anthracene Based Bright Aggregation-Induced Emission Organic Dots for Hela Cells Imaging
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用于 Hela 细胞成像的 9,10-（二乙烯基）蒽基明亮聚集诱导发射有机点
DOI：
10.1166/jnn.2020.17376
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发表时间：
2020
影响因子：
--
通讯作者：
Hua Li
中科院分区：
文献类型：
--
作者：
Yan Zhang;Na Liu;Shuang Su;Rui Jiang;Hua Li
荧光有机点已广泛用于各种生物成像。为了获得具有高量子产率（QY）的有机点，使用具有聚集诱导发射（AIE）性质的不同分子和具有两亲性的聚合物。将AIE分子9，10-二乙烯基蒽（DSA）分别用聚苯乙烯-马来酸酐共聚物（PSMA）和聚异丁烯-马来酸酐共聚物（PIMA）包覆，得到了新颖的AIE有机发光点。制备的AIE有机量子点（PSMA量子点和PIMA量子点）具有均匀的粒径分布、球形外观和小的粒径（约23 nm）。然而，PSMA点具有比PIMA点更高的QY，并且当PSMA：DSA的质量比为1：1时达到约28.9%。在叶酸（FA）缀合到PSMA点的表面之后，获得FA-点。FA-点具有均匀的粒径，良好的光学性能，稳定性和生物相容性。FA点可以标记叶酸受体过表达的细胞。体外细胞实验表明，FA-dots可被Hela细胞内吞，并对细胞进行特异性标记。
Fluorescent organic dots have been widely utilized for various biological imaging. To obtain organic dots with high quantum yield (QY), different molecules with aggregation-induced emission (AIE) property and polymers with amphiphilic are used. By encapsulating AIE molecule 9,10-divinyl anthracene (DSA) with poly(styrene-co-maleic anhydride) and poly(isobutylene-alt-maleic anhydride) (PSMA and PIMA), novel bright AIE organic dots were obtained. The prepared AIE organic dots (PSMA dots and PIMA dots) had uniform particle size distribution, spherical appearance and small particle size (about 23 nm). However, PSMA dots had higher QYs than PIMA dots, and reached about 28.9% when the mass ratio of PSMA:DSA was 1:1. After folic acid (FA) conjugating to the surface of the PSMA dots, FA-dots were obtained. FA-dots possessed uniform particle size, good optical properties, stability and biocompatibility. The FA-dots could mark the cells where folate receptors were over-expressing. In vitro cell experiment indicated that FA-dots could be endocytosed into Hela cells, and marked the cell with specific.