Behavioral circatidal rhythms require Bmal1 in Parhyale hawaiensis
Behavioral circatidal rhythms require Bmal1 in Parhyale hawaiensis
复制标题
DOI：
10.1016/j.cub.2023.03.015
复制
发表时间：
2023-05-22
期刊：
影响因子：
9.2
通讯作者：
Emery，Patrick
中科院分区：
文献类型：
--
作者：
Kwiatkowski，Erica R.;Schnytzer，Yisrael;Emery，Patrick
生活在潮间带的生物暴露在特别具有挑战性的环境中。除了光照强度的每日变化和光周期和天气模式的季节性变化外，它们还因潮汐而经历环境条件的剧烈振荡。为了预测潮汐，从而优化它们的行为和生理，占据潮间带生态位的动物已经获得了昼夜节律钟。虽然这些生物钟的存在早已为人所知，但其潜在的分子组成已被证明难以识别，这在很大程度上是因为缺乏适合遗传操作的潮间带模式生物。特别是，昼夜节律和昼夜分子钟之间的关系，以及共享遗传成分的可能性，一直是一个长期存在的问题。在这里，我们介绍了遗传上易处理的甲壳动物Parhyale hawaiensisas一个系统的昼夜节律的研究。首先，我们证明了P. hawaiensishibits鲁棒的12.4小时的运动节律，可以被夹带到人工潮汐方案和温度补偿。使用CRISPR-Cas9基因组编辑，我们证明了核心生物钟基因Bmal 1是昼夜节律所必需的。因此，我们的研究结果表明，Bmal 1是昼夜节律和昼夜节律钟之间的分子联系，并建立P. hawaiensis作为一个强大的系统来研究昼夜节律及其夹带的分子机制。
Organisms living in the intertidal zone are exposed to a particularly challenging environment. In addition to daily changes in light intensity and seasonal changes in photoperiod and weather patterns, they experience dramatic oscillations in environmental conditions due to the tides. To anticipate tides, and thus optimize their behavior and physiology, animals occupying intertidal ecological niches have acquired circatidal clocks. Although the existence of these clocks has long been known, their underlying molecular components have proven difficult to identify, in large part because of the lack of an intertidal model organism amenable to genetic manipulation. In particular, the relationship between the circatidal and circadian molecular clocks, and the possibility of shared genetic components, has been a long-standing question. Here, we introduce the genetically tractable crustaceanParhyale hawaiensisas a system for the study of circatidal rhythms. First, we show thatP. hawaiensisexhibits robust 12.4-h rhythms of locomotion that can be entrained to an artificial tidal regimen and are temperature compensated. Using CRISPR-Cas9 genome editing, we then demonstrate that the core circadian clock geneBmal1is required for circatidal rhythms. Our results thus demonstrate thatBmal1is a molecular link between circatidal and circadian clocks and establishP. hawaiensisas a powerful system to study the molecular mechanisms underlying circatidal rhythms and their entrainment.