Immunohistochemical demonstration of elevated expression of epidermal growth factor receptor in the neoplastic changes of cervical squamous epithelium
Immunohistochemical demonstration of elevated expression of epidermal growth factor receptor in the neoplastic changes of cervical squamous epithelium
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DOI：
10.1002/cncr.2820690519
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发表时间：
2010-08
期刊：
影响因子：
6.2
通讯作者：
T. Maruo;M. Yamasaki;C. Ladines-Llave;M. Mochizuki
中科院分区：
文献类型：
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作者：
T. Maruo;M. Yamasaki;C. Ladines-Llave;M. Mochizuki
为了评估表皮生长因子（EGF）受体在宫颈鳞状细胞上皮肿瘤形成过程中的表达，我们使用抗EGF受体单克隆抗体的亲和素-生物素免疫过氧化物酶技术检测了正常、癌前和恶性宫颈组织中EGF受体的存在。受体的主要染色在大多数天-塑性上皮和原位癌中观察到。在侵袭性鳞状癌中，不同组织学细胞类型的EGF受体免疫组化检测水平差异较大。大细胞非角化癌及其角化对应物含有高水平的EGF受体；小细胞非角化癌缺乏免疫染色的EGF受体，这些结果提示EGF受体的表达升高可能参与了宫颈鳞状上皮肿瘤发生的初始阶段，EGF受体的表达可能与宫颈鳞状上皮细胞的分化或去分化有关。
To evaluate epidermal growth factor (EGF) receptor expression in the neoplastic process of squamous cell epithelium of the uterine cervix, normal, premalignant, and malignant cervical tissues were examined for the presence of EGF receptor by the avidin‐biotin immunoperoxidase techniques with a monoclonal antibody to EGF receptor, Although normal cervical epithelium did not show appreciable staining for EGF receptor, predominant staining for the receptor was observed in most dys‐plastic epithelia and carcinomas in situ. In invasive squamous carcinoma, there was a great difference in the immunohistochemically detected levels of EGF receptor among the histologic cell types. Large cell nonkeratinizing carcinoma and its keratinizing counterpart contained high levels of EGF receptor; small cell nonkeratinizing carcinoma lacked immunostainable EGF receptor, These results suggest that the elevated expression of EGF receptor may be involved in the initial stage of tumori‐genesis of cervical squamous epithelium and that EGF receptor expression may be related to the differentiation or dedifferentiation of cervical squamous carcinoma cells.