Photosensitized production of singlet oxygen.
Photosensitized production of singlet oxygen.
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DOI：
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发表时间：
2000
影响因子：
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通讯作者：
I. Kochevar;R. Redmond
中科院分区：
文献类型：
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作者：
I. Kochevar;R. Redmond
光敏化是在溶液和细胞中产生单线态氧的一种简单可控的方法。描述了用于确定溶液中单线态氧的产率、用于测量单线态氧与底物之间的反应速率以及用于比较由细胞中的不同光敏剂产生的单线态氧的有效性的方法。这些定量测量可以更好地理解单线态氧与生物分子的相互作用。
Photosensitization is a simple and controllable method for the generation of singlet oxygen in solution and in cells. Methods are described for determining the yield of singlet oxygen in solution, for measurement of the rate of reaction between singlet oxygen and a substrate, and for comparing the effectiveness of singlet oxygen generated by different photosensitizers in cells. These quantitative measurements can lead to better understanding of the interaction of singlet oxygen with biomolecules.