Suitability of Capillary Blood for Quantitative Assessment of G6PD Activity and Performances of G6PD Point-of-Care Tests
Suitability of Capillary Blood for Quantitative Assessment of G6PD Activity and Performances of G6PD Point-of-Care Tests
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DOI：
10.4269/ajtmh.14-0696
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发表时间：
2015-04-01
影响因子：
3.3
通讯作者：
Nosten, Francois H.
中科院分区：
文献类型：
--
作者：
Bancone, Germana;Chu, Cindy S.;Nosten, Francois H.
伯氨喹和其他8-氨基喹啉用于消除疟疾的使用受到阻碍，除其他因素外，用于诊断葡萄糖-6-磷酸脱氢酶（G6 PD）缺乏症的护理点测试的可用性有限。从历史上看，G6 PD分析最常用的血液来源是静脉血，而现场使用的诊断器械需要使用毛细血管血;数据表明，两种血液来源在血红蛋白浓度和红细胞数量方面通常不同。因此，我们分析了从相同的健康供体抽取的毛细血管和静脉血中G6 PD活性的相关性，该相关性通过两种定性测试（荧光斑点测试和CareStart测试）与金标准定量分光光度法进行评估。对150例G6 PD表型正常、中等和缺乏的受试者的结果表明，尽管毛细血管和静脉血中上述特征之间存在差异，但这些差异不会影响血红蛋白浓度或红细胞计数校正后G6 PD活性的定量评估。此外，我们还评估了两种血液来源中残留酶活性的不同活性阈值下，两种定性检测对金标准分光光度法的灵敏度和特异性。
The use of primaquine and other 8-aminoquinolines for malaria elimination is hampered by, among other factors, the limited availability of point-of-care tests for the diagnosis of glucose-6-phosphate dehydrogenase (G6PD) deficiency. Historically, the most used source of blood for G6PD analyses is venous blood, whereas diagnostic devices used in the field require the use of capillary blood; data have shown that the two sources of blood often differ with respect to hemoglobin concentration and number of red blood cells. Therefore, we have analyzed, in both capillary and venous blood drawn from the same healthy donors, the correlation of G6PD activity assessed by two qualitative tests (the Fluorescent Spot test and the CareStart test) with the gold standard quantitative spectrophotometric assay. Results obtained on 150 subjects with normal, intermediate, and deficient G6PD phenotypes show that, although differences exist between the aforementioned characteristics in capillary and venous blood, these do not impact on the quantitative assessment of G6PD activity after corrected for hemoglobin concentration or red blood cell count. Furthermore, we have assessed the sensitivity and specificity of the two qualitative tests against the gold standard spectrophotometric assay at different activity thresholds of residual enzymatic activity in both blood sources.