RNA maps reveal new RNA classes and a possible function for pervasive transcription
RNA maps reveal new RNA classes and a possible function for pervasive transcription
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DOI：
10.1126/science.1138341
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发表时间：
2007-06-08
期刊：
影响因子：
56.9
通讯作者：
Gingeras, Thomas R.
中科院分区：
文献类型：
--
作者：
Kapranov, Philipp;Cheng, Jill;Gingeras, Thomas R.
真核基因组的显着部分产生RNA，其中大部分未经注释，并且蛋白质编码的潜力降低。在这项全基因组范围的研究中研究了基因组起源和人核和胞质聚腺苷酸化RNA的长度超过200个核苷酸（NT）和全细胞RNA小于200 nt。分配了存在于检测到的RNA中的核苷酸的亚细胞地址，并研究了它们的潜在处理为简短的RNA。综上所述，这些观察结果表明，一些未经注释的RNA作为短RNA的主要成绩单的新作用。已经确定了三种潜在的RNA类别类别，其中两个是同步保守的，并与蛋白质编码基因的表达状态相关。这些数据支持人类转录组的高度交错组织。
Significant fractions of eukaryotic genomes give rise to RNA, much of which is unannotated and has reduced protein-coding potential. The genomic origins and the associations of human nuclear and cytosolic polyadenylated RNAs longer than 200 nucleotides ( nt) and whole-cell RNAs less than 200 nt were investigated in this genome-wide study. Subcellular addresses for nucleotides present in detected RNAs were assigned, and their potential processing into short RNAs was investigated. Taken together, these observations suggest a novel role for some unannotated RNAs as primary transcripts for the production of short RNAs. Three potentially functional classes of RNAs have been identified, two of which are syntenically conserved and correlate with the expression state of protein-coding genes. These data support a highly interleaved organization of the human transcriptome.