A chromosome 11 YAC library.
A chromosome 11 YAC library.
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11 号染色体 YAC 文库。
DOI：
10.1006/geno.1993.1233
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发表时间：
1993
期刊：
影响因子：
4.4
通讯作者：
Shows,TB
中科院分区：
文献类型：
--
作者：
Qin,S;Zhang,J;Isaacs,CM;Nagafuchi,S;JaniSait,SN;Abel,KJ;Higgins,MJ;Nowak,NJ;Shows,TB
从J1细胞系构建了11号染色体的靶向酵母人工染色体（YAC）文库，该细胞系在仓鼠DNA背景中携带单个人11号染色体。在筛选过程中检测在文库构建过程中产生的种间嵌合克隆，并从文库中消除。使用这样的文库，重叠群组装变得不那么困难，因为复杂性降低，并且克隆插入物的末端可以被拯救到远离邻近克隆的地方。该文库含有> 1824个克隆，平均插入片段长度为337 kb。这代表了11号染色体的四倍覆盖率或从文库中回收独特单拷贝序列的> 95%的机会。200个YAC克隆通过荧光原位杂交定位，发现它们沿染色体沿着随机分布。该文库已经用11号染色体标记HBB、GLUR 4、H19和D11S193的探针进行了筛选。已经分离了每个基因座的相应YAC克隆。该分析表明文库是无偏的，同源YAC克隆可以用11号染色体标记回收，并且广泛的重叠群组装应该是可行的。
A targeted yeast artificial chromosome (YAC) library for chromosome 11 has been constructed from the J1 cell line that carries a single human chromosome 11 within a hamster DNA background. Interspecies chimeric clones generated during construction of the library were detected during the screening process and eliminated from the library. Contig assembly becomes much less difficult using such a library as the complexity is decreased and the ends of the clone inserts can be rescued far walking to neighboring clones. The library contains > 1824 clones with an average insert length of 337 kb. This represents a fourfold coverage of chromosome 11 or a > 95% chance of recovering a unique single-copy sequence from the library. Two hundred YAC clones were localized by fluorescencein situhybridization and found to be randomly distributed along the chromosome. The library has been screened with probes for the chromosome 11 markers HBB, GLUR4, H19, and D11S193. Corresponding YAC clones have been isolated for each locus. This analysis has indicated that the library is unbiased, that cognate YAC clones can be recovered with chromosome 11 markers, and that extensive contig assembly should be feasible.