The phytochrome gene family in grasses (Poaceae): A phylogeny and evidence that grasses have a subset of the loci found in dicot angiosperms
The phytochrome gene family in grasses (Poaceae): A phylogeny and evidence that grasses have a subset of the loci found in dicot angiosperms
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DOI：
10.1093/oxfordjournals.molbev.a025677
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发表时间：
1996-10-01
影响因子：
10.7
通讯作者：
Sharrock, RA
中科院分区：
文献类型：
--
作者：
Mathews, S;Sharrock, RA
光敏色素核基因家族编码感光蛋白，其在植物的整个生命中介导对红光和远红光的发育反应。根据双子叶开花植物拟南芥的研究，该家族已被建模为包括五个基因座，PHYA-PHYE。然而，最近的研究表明，拟南芥模型可能并不完全代表一些开花植物群体，因为与拟南芥PHYA和PHYB相关的其他PHY基因座显然在双子叶植物中独立进化了几次，而单子叶开花植物可能缺乏拟南芥PHYD和PHYE的直系同源物。尽管如此，光敏色素核苷酸数据在生物进化的研究中提供了信息，因为这些位点以单拷贝序列的形式出现，并且似乎是独立进化的。我们继续我们的调查光敏色素基因家族在开花植物中广泛取样的禾本科。对光敏色素核DNA数据进行了分支分析，以解决以下问题：（1）数据是否与光敏色素基因在单子叶植物和高等双子叶植物中的差异分布模式一致，在高等双子叶植物中存在PHYA，B，C，D和E的同源物，但在单子叶植物中只有PHYA，B和C，以及（2）它们在禾本科中揭示了什么样的系统发育模式？这些分析的结果，和Southern印迹实验，是一致的观察，光敏色素基因家族在禾本科植物包括在其他单子叶植物中检测到的位点的相同子集。此外，对于禾本科植物的有机体发育研究，这些数据显示出对其他数据集仅弱解决的关系提供了重要的支持。
The phytochrome nuclear gene family encodes photoreceptor proteins that mediate developmental responses to red and far red light throughout the life of the plant. From studies of the dicot flowering plant Arabidopsis, the family has been modeled as comprising five loci, PHYA-PHYE. However, it has been shown recently that the Arabidopsis model may not completely represent some flowering plant groups because additional PHY loci related to PHYA and PHYB of Arabidopsis apparently have evolved independently several times in dicots, and monocot flowering plants may lack orthologs of PHYD and PHYE of Arabidopsis. Nonetheless, the phytochrome nucleotide data were informative in a study of organismal evolution because the loci occur as single copy sequences and appear to be evolving independently. We have continued our investigation of the phytochrome gene family in flowering plants by sampling extensively in the grass family. The phytochrome nuclear DNA data were cladistically analyzed to address the following questions: (1) Are the data consistent with a pattern of differential distribution of phytochrome genes among monocots and higher dicots, with homologs of PHYA, B, C, D, and E present in higher dicots, but of just PHYA, B, and C in monocots, and (2) what phylogenetic pattern within Poaceae do they reveal? Results of these analyses, and of Southern blot experiments, are consistent with the observation that the phytochrome gene family in grasses comprises the same subset of loci detected in other monocots. Furthermore, for studies of organismal phylogeny in the grass family, the data are shown to provide significant support for relationships that are just weakly resolved by other data sets.