Genetic and transcriptional dissection of resistance to Claviceps purpurea in the durum wheat cultivar Greenshank
Genetic and transcriptional dissection of resistance to Claviceps purpurea in the durum wheat cultivar Greenshank
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DOI：
10.1007/s00122-020-03561-9
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发表时间：
2020-02-14
影响因子：
5.4
通讯作者：
Menzies, Jim G.
中科院分区：
文献类型：
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作者：
Gordon, Anna;McCartney, Curt;Menzies, Jim G.
在硬粒小麦品种绿柄麦中鉴定出4个麦角菌抗性QTL。麦角菌（Claviceps purpurea）是一种禾草病原体，它感染花，用麦角菌菌核取代种子。麦角菌是黑麦、大麦和小麦，特别是杂交种子生产系统中的一个重要问题。此外，有证据表明，积聚在菌核内的剧毒生物碱会交叉污染原本健康的谷物。寄主对紫锥虫的抗性是罕见的，抗性位点很少被发现。在本研究中，四个麦角抗性位点分别位于硬粒小麦cv的1B、2A、5A和5B染色体上。青足鹬。通过分析麦角菌感染相关表型，即产生菌核或导致子房死亡但无菌核的接种花的数量、产生的蜜露水平、总菌核重量和每穗平均菌核重量和大小，来评估麦角菌抗性。在加拿大和英国进行了麦角菌检测。主要影响QTL、qcp、aafc。DH-2A，在加拿大和英国的实验中都被检测到，对蜜露的生产水平有显著的影响。QCp.aafc。DH-5B对每穗总菌核重影响最大。QCp.aafc。DH-1B仅在加拿大实验和QCp.aafc中检测到。DH-5A在英国的实验。一项RNASeq分析显示，与四个麦角抗性QTL不同组合相关的小麦差异表达基因中，定位于QCp.aafc的deg数量不成比例。DH-1B QCp.aafc。DH-2A和QCp.aafc。DH-5B QTL间隔。
Key message Four QTL for ergot resistance (causal pathogen Claviceps purpurea) have been identified in the durum wheat cultivar Greenshank. Claviceps purpurea is a pathogen of grasses that infects flowers, replacing the seed with an ergot sclerotium. Ergot presents a significant problem to rye, barley and wheat, in particular hybrid seed production systems. In addition, there is evidence that the highly toxic alkaloids that accumulate within sclerotia can cross-contaminate otherwise healthy grain. Host resistance to C. purpurea is rare, few resistance loci having been identified. In this study, four ergot resistance loci are located on chromosomes 1B, 2A, 5A and 5B in the durum wheat cv. Greenshank. Ergot resistance was assessed through analysis of phenotypes associated with C. purpurea infection, namely the number of inoculated flowers that produced sclerotia, or resulted in ovary death but no sclerotia, the levels of honeydew produced, total sclerotia weight and average sclerotia weight and size per spike. Ergot testing was undertaken in Canada and the UK. A major effect QTL, QCp.aafc.DH-2A, was detected in both the Canadian and UK experiments and had a significant effect on honeydew production levels. QCp.aafc.DH-5B had the biggest influence on total sclerotia weight per spike. QCp.aafc.DH-1B was only detected in the Canadian experiments and QCp.aafc.DH-5A in the UK experiment. An RNASeq analysis, undertaken to identify wheat differentially expressed genes associated with different combinations of the four ergot resistance QTL, revealed a disproportionate number of DEGs locating to the QCp.aafc.DH-1B, QCp.aafc.DH-2A and QCp.aafc.DH-5B QTL intervals.