Skin carriage of acinetobacters in Hong Kong
Skin carriage of acinetobacters in Hong Kong
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DOI：
10.1128/jcm.37.9.2962-2967.1999
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发表时间：
1999-09-01
影响因子：
9.4
通讯作者：
Cheng, AFB
中科院分区：
文献类型：
--
作者：
Chu, YW;Leung, CM;Cheng, AFB
我们研究了不动杆菌属的携带。通过扩增核糖体 DNA 限制性分析，对 2 家医院 79 名患者的 5 个表层部位、133 名社区健康对照（医学生和新护士）以及 198 名不同班级的实习护士，对 201 名受试者的 364 个阳性部位和 124 份血培养分离株（1997 年至 1998 年）总共 431 株分离株进行了基因分种，基因种 3 是最多的。常见品种。实习护士（131 名中的 42 名）的携带率显着低于社区新护士（38 名中的 25 名）（卡方检验，P = 0.0004；比值比 [ORI，4.08；95% 置信限，1.78 至 9.41），但与同一医院的患者（42 名中的 20 名）没有显着差异（P = 0.1）。来自威尔士亲王医院的血培养物和受试者（急性患者和实习护士）的基因组彼此相似，但与来自社区或另一家医院的受试者不同（卡方检验，P < 0.0001）。至少两个位点呈阳性的受试者中有一半具有不同的基因种。在检查的 28 个位点中，通过随机扩增多态性 DNA 分析，68% 显示出相同基因种分离株之间的菌株变异。在 6 周或 6 个月后再次采集样本的 106 名受试者中，有一半仅一次呈阳性。在至少两次呈阳性的 17 名受试者中，每次都在 13 名受试者中产生不同的基因种。我们的结果表明，大多数健康受试者的皮肤携带具有低密度、基因种和品系变异、持续时间短以及特定地点的典型性等特点。
We studied the carriage of Acinetobacter spp. at five superficial sites in 79 patients from two hospitals, in 133 healthy controls from the community (medical students and new nurses), and in 198 student nurses in different classes, A total of 431 isolates fi om 364 positive sites of 201 subjects and 124 blood culture isolates (1997 to 1998) were genospeciated by amplified ribosomal DNA restriction analysis, Genospecies 3 was the most common species. The carriage rate of student nurses (42 of 131) was significantly lower than that of new nurses from the community (25 of 38) (chi-square test, P = 0.0004; odds ratio [ORI, 4.08; 95% confidence limits, 1.78 to 9.41) but not significantly different (P = 0.1) from that of patients in the same hospital (20 of 42). Genospecies from blood cultures and subjects (acute patients and student nurses) from Prince of Wales Hospital were similar to one another but different from subjects from the community or from another hospital (chi-square test, P < 0.0001). Half of the subjects who were positive at at least two sites had different genospecies, Of the 28 sites examined, 68% showed strain variation among isolates of the same genospecies by random amplified polymorphic DNA analysis. Half of the 106 subjects who had samples taken again within 6 weeks or 6 months later were positive only once. In the 17 subjects who were positive on at least two occasions, each occasion yielded different genospecies in 13 subjects. Our results indicate that skin carriage in the majority of healthy subjects is characterized by low density, variation in genospecies and strains, short-term duration, and the typicality of a given locality.