Angiotensin stimulation of bovine adrenocortical cell growth.
Angiotensin stimulation of bovine adrenocortical cell growth.
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血管紧张素刺激牛肾上腺皮质细胞生长。
DOI：
10.1073/pnas.74.12.5569
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发表时间：
1977
影响因子：
11.1
通讯作者：
M. H. Simonian
中科院分区：
文献类型：
--
作者：
G. Gill;C. Ill;M. H. Simonian
在牛肾上腺皮质细胞单层培养中研究了控制肾上腺皮质细胞增殖的因素。血管紧张素II刺激细胞增殖和[3 H]胸苷掺入DNA，半最大有效浓度为0.96 +/- 0.27 nM。观察到对血管紧张素III的敏感性相似，对血管紧张素I和十四肽肾素底物的敏感性降低。虽然对血管紧张素II的敏感性与成纤维细胞生长因子（1.5 nM半最大有效浓度）相当，但血管紧张素的最大效应低于成纤维细胞生长因子和血清。高浓度的胰岛素（1-10微米）也刺激[3 H]胸苷掺入DNA和细胞增殖。[Sar1血管紧张素II的竞争性拮抗剂血管紧张素II阻断血管紧张素II对DNA合成的刺激，但不影响成纤维细胞生长因子和胰岛素对DNA合成的刺激。促肾上腺皮质激素（ACTH）阻断血管紧张素II和成纤维细胞生长因子的刺激作用。血管紧张素II刺激类固醇生成的剂量-反应曲线与刺激[3 H]胸苷掺入DNA的剂量-反应曲线相似。在检查的七种细胞类型中，只有肾上腺皮质细胞对血管紧张素II有DNA合成的刺激反应。
Factors controlling proliferation of adrenocortical cells have been studied in monolayer cultures of bovine adrenocortical cells. Angiotensin II stimulated cell proliferation and [3H]thymidine incorporation into DNA with a half-maximal effective concentration of 0.96 +/- 0.27 nM. Similar sensitivity to angiotensin III with reduced sensitivity to angiotensin I and tetradecapeptide renin substrate was observed. Although sensitivity to angiotensin II was equivalent to that for fibroblast growth factor (1.5 nM half-maximal effective concentration), maximal effects of angiotensin were less than for fibroblast growth factor and serum. High concentrations of insulin (1-10 micrometer) also stimulated [3H]thymidine incorporation into DNA and cell proliferation. [Sar1,Ile5,Ile8]Angiotensin II, a competitive antagonist of angiotensin II, blocked angiotensin II stimulation of DNA synthesis but did not affect fibroblast growth factor and insulin stimulation of DNA synthesis. Corticotropin (ACTH) blocked the stimulatory effects of both angiotensin II and fibroblast growth factor. The dose-response curves for angiotensin II stimulation of steroidogenesis were similar to those for stimulation of [3H]thymidine incorporation into DNA. Among the seven cell types examined, only adrenocortical cells responded to angiotension II with stimulation of DNA synthesis.