QTLs for muscat flavor and monoterpenic odorant content in grapevine (Vitis vinifera L.)
QTLs for muscat flavor and monoterpenic odorant content in grapevine (Vitis vinifera L.)
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DOI：
10.1007/s11032-006-9016-3
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发表时间：
2006-09-01
影响因子：
3.1
通讯作者：
This, P.
中科院分区：
文献类型：
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作者：
Doligez, A.;Audiot, E.;This, P.
葡萄中麝香风味的遗传决定因素知之甚少，尽管这一性状对鲜食葡萄育种至关重要。因此，我们进行了搜索的QTL（数量性状位点）的麝香评分和浆果含量的三个主要的游离单萜醇可能涉及，芳樟醇，橙花醇和香叶醇，基于两年的措施。利用139个分散性好的SSR标记对174个杂交后代进行基因分型，构建了MTP 2687 -85（Olivette × Ribol）×麝香（Hamburg）的亲本和共有框架遗传图谱。雌性、雄性和共有框架图分别跨越935、1365和1267 cM。对于QTL检测，进行了简单和复合区间作图，以及非参数Kruskal-Wallis检验。麝香评分的QTL被发现连锁群（LG）1，5和7。对于三个ln转化的单萜含量，具有主要效应的QTL（解释总表型方差的17- 55%）被发现在两年内共定位在LG 5、雄性和共有图谱上。在LG 2上发现了一个额外的芳樟醇QTL，在雌性和共有图谱上，以及在LG 13上发现了橙花醇和香叶醇的其他共位QTL，在雄性和共有图谱上。这些额外的QTL具有较低的效应（9-25%）。这些结果的贡献麝香香气遗传决定的知识进行了讨论，以及它们的潜在用途标记辅助育种的芳香葡萄新品种。
Little is known about the genetic determinism of muscat flavor in grape, although this trait is of major importance for table grape breeding. We therefore performed a search for QTLs (Quantitative Trait Loci) of both muscat score and berry content in the three main free monoterpene alcohols potentially involved, linalool, nerol and geraniol, based on two years of measures. Parental and consensus framework genetic maps of the cross MTP2687-85 (Olivette x Ribol) x Muscat of Hamburg were built after genotyping the 174 offspring for 139 well-scattered SSR markers. The female, male and consensus framework maps spanned 935, 1365 and 1267 cM, respectively. For QTL detection, simple and composite interval mapping were performed, as well as non parametric Kruskal-Wallis tests. QTLs for muscat score were found on linkage groups (LGs) 1, 5 and 7. For the three ln-transformed monoterpene contents, QTLs with major effects (explaining 17-55 % of total phenotypic variance) were found to be colocated on LG 5, on the male and consensus maps in both years. One additional QTL was found for linalool on LG 2, on female and consensus maps, as well as other colocated ones for nerol and geraniol on LG 13, on male and consensus maps. These additional QTLs had lower effects (9-25%). The contribution of these results to the knowledge of muscat aroma genetic determinism is discussed, as well as their potential usefulness for marker assisted breeding of new aromatic grape varieties.