Enzymatic reactions in liposomes using the detergent-induced liposome loading method.
Enzymatic reactions in liposomes using the detergent-induced liposome loading method.
复制标题
使用去垢剂诱导的脂质体装载方法在脂质体中进行酶促反应。
DOI：
10.1016/s0005-2736(98)00210-7
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
P. Monnard
中科院分区：
文献类型：
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作者：
T. Oberholzer;Emmanuel Meyer;Irene Amato;Ariel Lustig;P. Monnard
微区隔化是生命起源的关键一步。30多年前，Oparin等人提出了基于所谓凝聚体中发生的生化反应的模型。他们的意图是开发可以建立半透性微室的系统。在目前的工作中，我们遵循他们的直觉，但我们使用表征良好的双层结构而不是表征不佳的凝聚。磷脂脂质体可以用作微反应器，但它们只有适度的渗透性，因此，在这些结构内发生的化学反应在相对较短的时间后就会耗尽。研究表明，即使是高度稳定的1-棕榈酰-2-油酰- cn -甘油-3-磷酸胆碱（POPC）脂质体，当用胆酸钠处理时，也可以用作半透性微反应器。利用这种混合脂质体，我们描述了脂质体内部发生的生化反应，而同样的反应在外部介质中被阻止。此外，我们表明，这种胆碱诱导的POPC双层通透性甚至可以用于从外部装载大分子，如酶。
Microcompartmentalization is a crucial step in the origin of life. More than 30 years ago, Oparin et al. proposed models based on biochemical reactions taking place in so-called coacervates. Their intention was to develop systems with which semipermeable microcompartments could be established. In the present work we follow their intuition, but we use well-characterized bilayer structures instead of the poorly characterized coacervates. Liposomes from phospholipids can be used as microreactors but they exhibit only a modest permeability and, therefore, chemical reactions occurring inside these structures are depleted after a relatively short period. Here it is shown that even highly stable liposomes from 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) can be used as semipermeable microreactors when treated with sodium cholate. Using this kind of mixed liposomes, we describe a biochemical reaction occurring inside the liposomes while the same reaction is prevented in the external medium. In addition, we show that this cholate-induced permeability of POPC bilayers can even be used to load macromolecules such as enzymes from the outside.