Internalization of ferromagnetic nanowires by different living cells.
Internalization of ferromagnetic nanowires by different living cells.
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DOI：
10.1186/1477-3155-4-9
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发表时间：
2006-09-05
影响因子：
10.2
通讯作者：
Coey JM
中科院分区：
文献类型：
--
作者：
Prina-Mello A;Diao Z;Coey JM
活细胞，无论是贴壁的或悬浮的，内化镍纳米线的能力被证明对MC3T3-E1，UMR106-肿瘤和骨髓基质细胞。采用电沉积法制备了长20μm、直径200 nm的纳米线。实现了三种细胞类型的细胞分离和操作。外加磁场成功地定向了内化纳米线，但在贴壁细胞上没有诱导出明显的各向异性。纳米线倾向于与细胞质金属蛋白结合，并在细胞核周围触发溶酶体重组。这项工作展示了纳米线在贴壁细胞和悬浮细胞中用于细胞分离和操作的应用，并进一步探讨了它们在纳米生物技术中的作用。
The ability of living cells, either adherent or suspended, to internalize nickel nanowires is demonstrated for MC3T3-E1, UMR106-tumour and Marrow-Stromal cells. Nanowires were produced by electrodeposition, 20 μm long and 200 nm in diameter. Cell separation and manipulation was achieved for the three cell types. Applied magnetic field successfully oriented the internalized nanowires but no clear anisotropy is induced on the adherent cells. Nanowires tend to bind to cytoplasm metalloproteins and trigger lysosome reorganization around the nucleus. This work demonstrates the applications of nanowires in adherent and suspended cells for cell separation and manipulation, and further explore into their role in nanobiotechnology.