Orientation of carotenoid molecules in the eyespot of alga:: In situ polarized resonance raman spectroscopy
Orientation of carotenoid molecules in the eyespot of alga:: In situ polarized resonance raman spectroscopy
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DOI：
10.1366/0003702001950832
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发表时间：
2000-08-01
影响因子：
3.5
通讯作者：
Tsuboi, M
中科院分区：
文献类型：
--
作者：
Kubo, Y;Ikeda, T;Tsuboi, M
用514.5 nm激发测量了裸藻和衣藻单细胞内不同点的拉曼光谱。在每个点上，在1530和1159 cm(-1)处都出现了两条可归属于类胡萝卜素的强带。利用拉曼作图系统，绘制了1530 cm(-1)处的拉曼强度与代表细胞内某个位置的(x，y)坐标的关系。结果表明，对于所研究的两种藻类，眼斑的类胡萝卜素含量显著较高，少量类胡萝卜素均匀地分布在叶绿体中。测图系统的空间分辨率高于1微米，衣藻眼斑呈1微米×2微米的细长形状。利用偏振器，发现衣藻眼斑中的类胡萝卜素链沿其长轴排列，与体轴平行。
Raman spectra of single cells of Euglena and Chlamydomonas have been examined with 514.5 nm excitation at various points within the cells. At every point, two strong bands, which are assignable to carotenoid, appeared at 1530 and 1159 cm(-1). By the use of a Raman mapping system, the Raman intensity at 1530 cm(-1) has been plotted against the (x,y) coordinate representing a location within the cell, It has been shown that, for both algae examined, the eyespot has a prominently high carotenoid content, and a small amount of carotenoid is uniformly distributed among the chloroplast. The spatial resolution of the mapping system has been shown to be higher than 1 mu m, and the Chlamydomonas eyespot has an elongated shape of 1 mu m x 2 mu m. By use of a polarizer, the carotenoid chains in the Chlamydomonas eyespot have been found to be aligned along its long axis, which is parallel to the body axis.