Telomere shortening in Barrett's mucosa and esophageal adenocarcinoma and its association with loss of heterozygosity
Telomere shortening in Barrett's mucosa and esophageal adenocarcinoma and its association with loss of heterozygosity
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DOI：
10.1080/00365520902718705
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发表时间：
2009-01-01
影响因子：
1.9
通讯作者：
Takubo, Kaiyo
中科院分区：
文献类型：
--
作者：
Shiraishi, Hiroaki;Mikami, Tetuo;Takubo, Kaiyo
Objective.端粒缩短被认为与遗传不稳定性有关。本研究的目的是测量端粒长度在一系列巴雷特腺癌（BA），重点是端粒/着丝粒荧光强度比（TCR）与组织定量荧光原位杂交（Q-FISH）。材料和方法。对11例BA的上食管（UE）、下食管（LE）、Barrett粘膜（BM）、BA和贲门粘膜（GC）进行了评价。用端粒和着丝粒两种特异性肽核酸探针进行Q-FISH。用CCD照相机和原始软件（Tissue Telo）分析切片以测量TCR。此外，还实施了激光捕获显微切割和基因扫描来评估遗传不稳定性。结果BM和BA（在较小程度上）的TCR值显著低于其他组织，特别是杂合性（洛）阳性病例。然而，微卫星不稳定性（MSI）阳性组和阴性组之间无显著差异。结论.在我们对BA系列的研究中，端粒长度似乎随着组织学异常的程度而改变，其减少与洛缺失有关。
Objective. Telomere shortening is thought to be associated with genetic instability. The purpose of this study was to measure telomere length in a series of Barrett's adenocarcinomas (BAs), focusing on the telomere/centromere fluorescent intensity ratio (TCR) with tissue quantitative fluorescent in situ hybridization (Q-FISH). Material and methods. A total of 11 cases of BA were evaluated for upper esophagus (UE), lower esophagus (LE), Barrett's mucosa (BM), BA, and gastric cardiac mucosa (GC). Q-FISH was performed using two kinds of peptide nucleic acid probe, specific for telomeres and centromeres. The sections were analyzed with a CCD camera and original software (Tissue Telo) for measuring TCR. In addition, Laser Capture Microdissection and GeneScan were implemented for evaluation of genetic instability. Results. The TCR values in BM and, to a lesser extent, BA were significantly lower than those in the other tissues, particularly in heterozygosity (LOH)-positive cases. However, no significant difference was evident between microsatellite instability (MSI)-positive and -negative groups. Conclusions. In our study of BA series, telomere length appeared to change with the degree of histological atypia, with decreases linked to LOH.