Deep Profiling of the Proteome Dynamics of Pseudomonas aeruginosa Reference Strain PAO1 under Different Growth Conditions.
Deep Profiling of the Proteome Dynamics of Pseudomonas aeruginosa Reference Strain PAO1 under Different Growth Conditions.
复制标题
DOI：
10.1021/acs.jproteome.2c00785
复制
发表时间：
2023-05
影响因子：
4.4
通讯作者：
M. Hou;Jingnan Huang;Tianyuan Jia;Yudong Guan;Fan Yang;Hongchao Zhou;Piying Huang;Jigang Wang;Liang Yang;Li Dai
中科院分区：
文献类型：
--
作者：
M. Hou;Jingnan Huang;Tianyuan Jia;Yudong Guan;Fan Yang;Hongchao Zhou;Piying Huang;Jigang Wang;Liang Yang;Li Dai
铜绿假单胞菌是引起医院感染最常见的细菌之一，对各种条件都有很强的适应能力。在这里，我们使用独立于数据采集的基于定量蛋白质组学的方法，分析了铜绿假单胞菌参考菌株PAO1中不同生长阶段3489种蛋白质的丰度动态。在浮游生长过程中差异表达的蛋白质表现出几种不同的表达模式，并与各种生物过程相关，突出了PAO1蛋白质组在从加速期向稳定期的转变过程中的持续适应。通过比较生物膜和浮游细胞中的蛋白质表达，证实了T6SS、吩嗪生物合成、群体感应和c-di-GMP信号在生物膜形成过程中的已知作用。此外，我们还发现了几种可能在生物膜形成过程中发挥作用的新功能蛋白。最后，我们证明了不同生长状态操纵子内蛋白质表达的总体一致性，这使得研究共表达的蛋白质单位，以及反过来，研究操纵子结构中的调节成分。综上所述，我们提供了关于铜绿假单胞菌参考菌株PAO1蛋白质组学动力学的高质量和有价值的资源，并有可能促进我们对假单胞菌总体生理学的理解。
As one of the most common bacterial pathogens causing nosocomial infections, Pseudomonas aeruginosa is highly adaptable to survive under various conditions. Here, we profiled the abundance dynamics of 3489 proteins across different growth stages in the P. aeruginosa reference strain PAO1 using data-independent acquisition-based quantitative proteomics. The proteins differentially expressed during the planktonic growth exhibit several distinct patterns of expression profiles and are relevant to various biological processes, highlighting the continuous adaptation of the PAO1 proteome during the transition from the acceleration phase to the stationary phase. By contrasting the protein expressions in a biofilm to planktonic cells, the known roles of T6SS, phenazine biosynthesis, quorum sensing, and c-di-GMP signaling in the biofilm formation process were confirmed. Additionally, we also discovered several new functional proteins that may play roles in the biofilm formation process. Lastly, we demonstrated the general concordance of protein expressions within operons across various growth states, which permits the study of coexpression protein units, and reversely, the study of regulatory components in the operon structure. Taken together, we present a high-quality and valuable resource on the proteomic dynamics of the P. aeruginosa reference strain PAO1, with the potential of advancing our understanding of the overall physiology of Pseudomonas bacteria.