Direct determination of gentamicin components by capillary electrophoresis with potential gradient detection
Direct determination of gentamicin components by capillary electrophoresis with potential gradient detection
复制标题
DOI：
10.1002/elps.200406121
复制
发表时间：
2005
期刊：
影响因子：
2.9
通讯作者：
Lingling Yuan;Hongping Wei;Sam F. Y. Li
中科院分区：
文献类型：
--
作者：
Lingling Yuan;Hongping Wei;Sam F. Y. Li
建立了一种无需衍生化直接测定非紫外光活性物质的简便、快速的方法。用毛细管区带电泳-电位梯度检测法对氨基糖苷类抗生素混合物中的主要成分进行了检测，证明了该方法的有效性。在优化的分离条件下(0.2 mM十六烷基三甲基溴化铵(CTAB)，1 mM柠檬酸铵，pH 3.5)，庆大霉素在15min内可分离出3个主峰(C1、C1a和C2+C2a)。与其他毛细管电泳法相比，该方法对未衍生化庆大霉素具有更高的灵敏度。线性范围为10~500ppm。该方法具有良好的重复性和简便性，可替代美国药典和欧洲药典现行的含量测定方法。
A simple and fast method was developed to determine non‐UV active compounds directly without derivatization. The usefulness of the method was demonstrated by detecting the major components in aminoglycoside antibiotic mixtures using capillary zone electrophoresis with potential gradient detection. Under optimized separation conditions (0.2 mM cetyltrimethylammonium bromide (CTAB), 1 mM ammonium citrate, pH 3.5), gentamicin was separated into three major peaks (C1, C1a, and C2+C2a) within 15 min. This method showed better sensitivity than other capillary electrophoresis (CE) methods for determining underivatized gentamicin. The linear range was from 10 to 500 ppm. Because of its good repeatability and simplicity, this new method could be a good alternative for the current assays given by US Pharmacopoeia and European Pharmacopoeia.