Trapping effect on a small molecular drug with vascular-disrupting agent CA4P in rodent H22 hepatic tumor model: in vivo magnetic resonance imaging and postmortem inductively coupled plasma atomic emission spectroscopy
Trapping effect on a small molecular drug with vascular-disrupting agent CA4P in rodent H22 hepatic tumor model: in vivo magnetic resonance imaging and postmortem inductively coupled plasma atomic emission spectroscopy
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DOI：
10.3109/1061186x.2014.1002789
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发表时间：
2015-06-01
影响因子：
4.5
通讯作者：
Zhang, Jian
中科院分区：
文献类型：
--
作者：
Gao, Meng;Yao, Nan;Zhang, Jian
本研究的目的是验证combretastatin A-4-phosphate （CA4P）对小分子药物在啮齿动物肿瘤中的捕获作用。H22肝癌小鼠随机分为A组和b组。以T1WI、T2WI、DWI磁共振成像（MRI）为基线。A组小鼠注射Gd-DTPA和PBS。B组小鼠注射Gd-DTPA和CA4P。各组小鼠分别于0 h、3 h、6 h、12 h、24 h进行CE-T1WI治疗，比较两组小鼠CE-T1WI增强效果，计算信噪比（SNR）。比较两组肿瘤中ICP-AES测定的钆浓度。在早期CE-T1WI上，两组肿瘤同样增强。在延迟CE-T1WI上，A组的增强作用弱于B组。给药后6 h、12 h、24 h， A组的信噪比和肿瘤内钆浓度均低于B组。本研究表明，CA4P可以改善Gd-DTPA在肿瘤中的滞留，MRI可以动态监测CA4P作为小分子药物对Gd-DTPA局部滞留的捕获作用。
The aim of the present study is to verify the trapping effect of combretastatin A-4-phosphate (CA4P) on small molecular drugs in rodent tumors. Mice with H22 hepatocarcinoma were randomized into groups A and B. Magnetic resonance imaging (MRI) of T1WI, T2WI, and DWI was performed as baseline. Mice in group A were injected with Gd-DTPA and PBS. Mice in group B were injected with Gd-DTPA and CA4P. All mice undergo CE-T1WI at 0 h, 3 h, 6 h, 12 h, and 24 h. Enhancing efficacy of the two groups on CE-T1WI was compared with the signal-to-noise ratio (SNR) calculated. Concentrations of gadolinium measured by ICP-AES in the tumor were compared between groups. On the early CE-T1WI, tumors were equally enhanced in both groups. On the delayed CE-T1WI, the enhancing effect of group A was weaker than that of group B. The SNR and the concentration of gadolinium within the tumor of group A were lower than that of group B at 6 h, 12 h, and 24 h after administration. This study indicates that CA4P could improve the retention of Gd-DTPA in the tumor and MRI allowed dynamically monitoring trapping effects of CA4P on local retention of Gd-DTPA as a small molecular drug.