Molecular investigation of carbapenem resistance among multidrug-resistant Pseudomonas aeruginosa isolated clinically in Thailand
Molecular investigation of carbapenem resistance among multidrug-resistant Pseudomonas aeruginosa isolated clinically in Thailand
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DOI：
10.1111/1348-0421.12021
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发表时间：
2013-03-01
影响因子：
2.6
通讯作者：
Chomnawang, Mullika Traidej
中科院分区：
文献类型：
--
作者：
Khuntayaporn, Piyatip;Montakantikul, Preecha;Chomnawang, Mullika Traidej
从泰国三级医院的多重耐药（CR-MDR）微生物中分离出碳青霉烯类耐药铜绿假单胞菌。oprD mRNA表达下降（93.65%），mexAB-oprM mRNA表达上升（92.06%），mexXY mRNA表达上升（63.49%）。有趣的是，23 126（18.25%）菌株对亚胺培南的oprD表达下调和非上调mexCD-oprJ mRNA表达的敏感。产金属-内酰胺酶明确阳性24株（18.46%），弱阳性12株（9.23%）。在这两组分离株中，鉴定出imp-1、imp-14和Vim-2。产AmpC -内酰胺酶阳性率最低（3.97%）。得出的结论是，泰国的CR-MDR铜绿假单胞菌临床分离株具有多因素耐药机制。
Carbapenem resistant Pseudomonas aeruginosa were isolated among multidrug-resistant (CR-MDR) organisms from tertiary hospitals in Thailand. Decreased expression of oprD mRNA (93.65%) was predominant followed by increased expression of mexAB-oprM mRNA (92.06%) and mexXY mRNA (63.49%). Interestingly, 23 of 126 (18.25%) isolates were susceptible to imipenem with down-regulated oprD expression and non-up-regulated mexCD-oprJ mRNA expression. Metallo--lactamases production was clearly positive in 24 isolates (18.46%) and weakly positive in 12 isolates (9.23%). Among both of these sets of isolates, imp-1, imp-14 and vim-2 were identified. Hyperproduction of AmpC -lactamase had the lowest prevalence rate (3.97%). It was concluded that CR-MDR P. aeruginosa clinical isolates in Thailand possess multifactorial resistance mechanisms.