In vitro study of the impact of mechanical tension on the dermal fibroblast phenotype in the context of skin wound healing
In vitro study of the impact of mechanical tension on the dermal fibroblast phenotype in the context of skin wound healing
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DOI：
10.1016/j.jbiomech.2014.07.015
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发表时间：
2014-11-07
影响因子：
2.4
通讯作者：
Humbert, Philippe
中科院分区：
文献类型：
--
作者：
Rolin, Gwenael;Binda, Delphine;Humbert, Philippe
皮肤伤口愈合受到基质合成和降解的精细调节，基质合成和降解受真皮成纤维细胞活性支配。实际上，成纤维细胞合成许多细胞外基质蛋白（即，胶原）、重塑酶及其抑制剂。此外，它们分化成肌成纤维细胞，并能够在伤口部位产生内源性力。这种力在皮肤伤口愈合期间是至关重要的，并且已经被广泛研究。然而，很少有研究集中在外源性机械张力对真皮成纤维细胞表型的影响，这是本文的目的。为此，外源性的，定义的，循环和单轴机械应变施加到培养成纤维细胞作为刮伤单层。结果表明，成纤维细胞的反应的特点是增加的前胶原I型和TIMP-1的合成，和MMP-1的合成减少。划痕损伤单层的监测没有显示出任何减少动力学的填充时，施加机械张力。增殖的成纤维细胞和融合的单层细胞获得的其他结果表明，机械张力诱导的成纤维细胞的反应取决于他们的培养条件。总之，机械张力导致真皮成纤维细胞的分化，并可能增加其伤口愈合能力。因此，本研究中报道的外源性单轴和周期性机械张力可以被考虑用于促进皮肤伤口愈合。(C)2014爱思唯尔有限公司版权所有。
Skin wound healing is finely regulated by both matrix synthesis and degradation which are governed by dermal fibroblast activity. Actually, fibroblasts synthesize numerous extracellular matrix proteins (i.e., collagens), remodeling enzymes and their inhibitors. Moreover, they differentiate into myofibroblasts and are able to develop endogenous forces at the wound site. Such forces are crucial during skin wound healing and have been widely investigated. However, few studies have focused on the effect of exogenous mechanical tension on the dermal fibroblast phenotype, which is the objective of the present paper. To this end, an exogenous, defined, cyclic and uniaxial mechanical strain was applied to fibroblasts cultured as scratch-wounded monolayers. Results showed that fibroblasts' response was characterized by both an increase in procollagen type-I and TIMP-1 synthesis, and a decrease in MMP-1 synthesis. The monitoring of scratch-wounded monolayers did not show any decrease in kinetics of the filling up when mechanical tension was applied. Additional results obtained with proliferating fibroblasts and confluent monolayer indicated that mechanical tension-induced response of fibroblasts depends on their culture conditions. In conclusion, mechanical tension leads to the differentiation of dermal fibroblasts and may increase their wound-healing capacities. So, the exogenous uniaxial and cyclic mechanical tension reported in the present study may be considered in order to improve skin wound healing. (C) 2014 Elsevier Ltd. All rights reserved.