Gangliogenesis in leech: morphogenetic processes leading to segmentation in the central nervous system.
Gangliogenesis in leech: morphogenetic processes leading to segmentation in the central nervous system.
复制标题
水蛭的神经节生成：导致中枢神经系统分割的形态发生过程。
DOI：
10.1007/s004270050150
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Weisblat,DA
中科院分区：
文献类型：
--
作者：
Shain,DH;Ramirez-Weber,FA;Hsu,J;Weisblat,DA
使用细胞内的谱系示踪剂研究的主要神经原谱系（N系）的glossiphoniid水蛭胚胎，我们的特点是事件导致连续列的节段创始人细胞（NF和NS原胚细胞）离散，节段迭代神经节。预期神经节之间的分离首先表现为nf-的后边界和ns-衍生后代的前边界之间的裂缝。我们还确定了nf衍生细胞的亚系，这些亚系为每个节段提供了平行的细胞条纹。这些细胞条纹从每个新生神经节的背外侧边缘向腹侧突出到腹侧体壁。条纹的位置和方向表明，它们在形成后段神经中发挥作用;它们与神经节边界不一致，并且它们在神经节原基分离后形成。先前的研究表明，前条纹中的细胞表达leechangrailed类基因。因此，与果蝇中表达angrailed类基因的细胞的作用相反，水蛭中表达angrailed类基因的N-衍生细胞的条纹似乎在分节或分节极性中不起直接作用。
Using intracellular lineage tracers to study the main neurogenic lineage (N lineage) of the glossiphoniid leech embryo, we have characterized events leading from continuous columns of segmental founder cells (nf and ns primary blast cells) to discrete, segmentally iterated ganglia. The separation between prospective ganglia was first evident as a fissure between the posterior boundary of nf- and the anterior boundary of ns-derived progeny. We also identified the sublineages of nf-derived cells that contribute parallel stripes of cells to each segment. These stripes of cells project ventrolaterally from the dorsolateral margin of each nascent ganglion to the ventral body wall. The position and orientation of the stripes suggests that they play a role in forming the posterior segmental nerve; they are not coincident with the ganglionic boundary, and they form well after the separation of ganglionic primordia. Previous work has shown that cells in the anterior stripe express the leechengrailed-class gene. Thus, in contrast to the role of cells expressingengrailedinDrosophila, the stripes of N-derived cells expressing anengrailed-class gene in leech do not seem to play a direct role in segmentation or segment polarity.