A microchip-based multianalyte assay system for the assessment of cardiac risk
A microchip-based multianalyte assay system for the assessment of cardiac risk
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DOI：
10.1021/ac011150a
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发表时间：
2002-07-01
影响因子：
7.4
通讯作者：
McDevitt, JT
中科院分区：
文献类型：
--
作者：
Christodoulides, N;Tran, M;McDevitt, JT
据报道，一种以心脏为主题的新型基于芯片的多分析物检测系统的开发。这项工作是在“电子味觉芯片”的初步报告之后进行的，其中检测和定量了多种溶液相分析物，例如酸、碱、金属阳离子和生物辅因子。新设计的“心脏芯片”采用了一种几何形状，允许在微机械加工的凹坑中分离和捕获单个聚合物球，同时通过微流体结构向每个珠子快速引入一系列试剂/洗涤剂。这些小型化组件的组合有助于使用小样本量在短时间内完成复杂的测定。来自单珠的光信号用于完成免疫学测试，从而产生出色的分析特性。这里展示了这种新方法的强大功能和实用性，可同时检测人血清样本中的心脏危险因素、C 反应蛋白和白细胞介素 6。这次演示代表了开发有用的心脏芯片的第一步，该芯片可同时针对多种风险因素，并且可以针对特定的临床环境轻松定制。
The development of a novel chip-based multianalyte detection system with a cardiac theme is reported. This work follows the initial reports of "electronic taste chips" whereby multiple solution-phase analytes such as acids, bases, metal cations, and biological cofactors were detected and quantitated. The newly fashioned "cardiac chip" exploits a geometry that allows for isolation and entrapment of single polymeric spheres in micromachined pits while providing to each bead the rapid introduction of a series of reagents/washes through microfluidic structures. The combination of these miniaturized components fosters the completion of complex assays with short analysis times using small sample volumes. Optical signals derived from single beads are used to complete immunological tests that yield outstanding assay characteristics. The power and utility of this new methodology is demonstrated here for the simultaneous detection of the cardiac risk factors, C-reactive protein and interleukin-6, in human serum samples. This demonstration represents the first important step toward the development of a useful cardiac chip that targets numerous risk factors concurrently and one that can be customized readily for specific clinical settings.