The catabolism of amino acids to long chain and complex alcohols in Saccharomyces cerevisiae
The catabolism of amino acids to long chain and complex alcohols in Saccharomyces cerevisiae
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DOI：
10.1074/jbc.m211914200
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发表时间：
2003-03-07
影响因子：
4.8
通讯作者：
Hewlins, MJE
中科院分区：
文献类型：
--
作者：
Dickinson, JR;Eshantha, L;Hewlins, MJE
用~（13）C NMR谱和气相色谱-质谱联用技术研究了苯丙氨酸催化生成2-苯基乙醇和色氨酸催化生成乙醇的反应。苯丙氨酸和色氨酸首先脱氨基（分别为3-苯丙酮酸和3-吲哚丙酮酸），然后脱羧。这种脱羧作用可以通过Pdc 1 p、Pdc 5 p、Pdc 6p或Ydr 380 wp中的任何一种来实现; Yd 1080 cp在这两种氨基酸的催化作用中没有作用。我们还报道了亮氨酸催化酶Ydr 380 wp是次要的脱羧酶。因此，迄今为止研究的所有氨基酸分解代谢途径都使用来自五元家族的脱羧酶的细微不同谱，该五元家族包括Pdc 1 p、Pdc 5 p、Pdc 6p、Yd 1080 cp和Ydr 380 wp。使用含有影响七个AAD基因（推定的芳基醇脱氢酶）、五个ADH基因和SFA 1的所有可能的突变组合的菌株，表明氨基酸催化剂的最后步骤（醛转化为长链或复合醇）可以通过任何一种乙醇脱氢酶（Adh 1 p、Adh 2 p、Adh 3 p、Adh 4p、Adh 5 p）或Sfa 1 p（甲醛脱氢酶）完成。
The catabolism of phenylalanine to 2-phenylethanol and of tryptophan to tryptophol were studied by C-13 NMR spectroscopy and gas chromatography-mass spectrometry. Phenylalanine and tryptophan are first deaminated (to 3-phenylpyruvate and 3-indolepyruvate, respectively) and then decarboxylated. This decarboxylation can be effected by any of Pdc1p, Pdc5p, Pdc6p, or Ydr380wp; Yd1080cp has no role in the catabolism of either amino acid. We also report that in leucine catabolism Ydr380wp is the minor decarboxylase. Hence, all amino acid catabolic pathways studied to date use a subtly different spectrum of decarboxylases from the five-membered family that comprises Pdc1p, Pdc5p, Pdc6p, Yd1080cp, and Ydr380wp. Using strains containing all possible combinations of mutations affecting the seven AAD genes (putative Aryl alcohol dehydrogenases), five ADH genes, and SFA1, showed that the final step of amino acid catabolism (conversion of an aldehyde to a long chain or complex alcohol) can be accomplished by any one of the ethanol dehydrogenases (Adh1p, Adh2p, Adh3p, Adh4p, Adh5p) or by Sfa1p (formaldehyde dehydrogenase.).