REGULATION OF COLLAGEN-SYNTHESIS BY ASCORBIC-ACID
REGULATION OF COLLAGEN-SYNTHESIS BY ASCORBIC-ACID
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DOI：
10.1073/pnas.78.5.2879
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
PINNELL, SR
中科院分区：
文献类型：
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作者：
MURAD, S;GROVE, D;PINNELL, SR
经过长时间暴露于抗坏血酸，在培养的人皮肤成纤维细胞的胶原蛋白合成增加约8倍，无显着变化的非胶原蛋白的合成。抗坏血酸的这种作用似乎与其在胶原羟化中的辅因子功能无关。胶原蛋白分泌的情况下添加抗坏血酸是正常的羟赖氨酸，但轻度缺乏羟脯氨酸。在平行实验中，赖氨酸羟化酶（肽基赖氨酸，2-酮戊二酸：氧5-氧化还原酶，EC 1.14.11.4）活性响应于抗坏血酸施用而增加3倍;脯氨酸羟化酶（脯氨酰-甘氨酰-肽，2-酮戊二酸：氧氧化还原酶，EC 1.14.11.2）活性显著降低。显然，胶原多肽的合成、翻译后羟化和2种羟化酶的活性独立地受抗坏血酸的调节。
After prolonged exposure to ascorbate, collagen synthesis in cultured human skin fibroblasts increased approximately 8-fold with no significant change in synthesis of noncollagen protein. This effect of ascorbate appears to be unrelated to its cofactor function in collagen hydroxylation. The collagenous protein secreted in the absence of added ascorbate was normal in hydroxylysine but was mildly deficient in hydroxyproline. In parallel experiments, lysine hydroxylase (peptidyllysine, 2-oxoglutarate:oxygen 5-oxidoreductase, EC 1.14.11.4) activity increased 3-fold in response to ascorbate administration; proline hydroxylase (prolyl-glycyl-peptide, 2-oxoglutarate:oxygen oxidoreductase, EC 1.14.11.2) activity decreased considerably. Apparently, collagen polypeptide synthesis, posttranslational hydroxylations and activities of the 2 hydroxylases are independently regulated by ascorbate.