Gene expression analysis of strawberry achene and receptacle maturation using DNA microarrays
Gene expression analysis of strawberry achene and receptacle maturation using DNA microarrays
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DOI：
10.1093/jxb/erf026
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发表时间：
2002-10-01
影响因子：
6.9
通讯作者：
O'Connell, AP
中科院分区：
文献类型：
--
作者：
Aharoni, A;O'Connell, AP
采用大规模、单次测序和使用 DNA 微阵列的平行基因表达分析来全面研究草莓果实的成熟。从草莓（Fragariaxananassa）成熟果实cDNA文库中获得的总共1701个cDNA克隆（包括1100个草莓EST和601个未测序的cDNA）被展示在微阵列上，并用于监测花托和瘦果组织中同时的基因表达。表达比率分析确定了 259 个瘦果相关克隆中的 66 个 (25%) 和 182 个花托相关克隆中的 80 个 (44%)，两种组织类型之间的表达差异超过 4 倍。一半的瘦果相关基因假定编码具有未知功能的蛋白质，而其余的大部分是预测形成与瘦果成熟以及获得应激和干燥耐受性相关的信号和调控级联的一部分的蛋白质。其中包括磷酸酶、蛋白激酶、14-3-3 蛋白、转录因子等。在容器中，鉴定出可能与成熟相关的关键过程和新基因。推测编码与应激、细胞壁、DNA/RNA/蛋白质和初级代谢相关的蛋白质的基因具有高度代表性。除了提供对正在发育的草莓的基因表达程序和代谢途径的全球观察之外，这项研究还为分子育种方法的基因发现、启动子选择和标记提供了一个大型数据库和独特信息。
Large-scale, single pass sequencing and parallel gene expression analysis using DNA microarrays were employed for the comprehensive investigation of ripening in strawberry fruit. A total of 1701 cDNA clones (comprising 1100 strawberry ESTs and 601 unsequenced cDNAs) obtained from a strawberry (Fragariaxananassa) ripe fruit cDNA library were displayed on microarrays, and used for monitoring concurrent gene expression in receptacle and achene tissues. Analysis of expression ratios identified 66 out of the 259 (25%) achene-related clones and 80 out of 182 (44%) receptacle-related clones with more than a 4-fold difference in expression between the two tissue types. Half of the achene-associated genes putatively encode proteins with unknown function, and a large number of the remainder were proteins predicted to form part of the signal and regulation cascades related to achene maturation and acquisition of stress and desiccation tolerance. These included phosphatases, protein kinases, 14-3-3 proteins, transcription factors, and others. In the receptacle, key processes and novel genes that could be associated with ripening were identified. Genes putatively encoding proteins related to stress, the cell wall, DNA/RNA/protein, and primary metabolism were highly represented. Apart from providing a global observation on gene expression programmes and metabolic pathways in the developing strawberry, this study has made available a large database and unique information for gene discovery, promoter selection and markers for molecular breeding approaches.