Chronic perfluorooctanesulfonic acid exposure disrupts lipid metabolism in zebrafish
Chronic perfluorooctanesulfonic acid exposure disrupts lipid metabolism in zebrafish
复制标题
慢性全氟辛烷磺酸暴露会扰乱斑马鱼的脂质代谢
DOI：
10.1177/0960327116646615
复制
发表时间：
2017-03-01
影响因子：
2.8
通讯作者：
Yang, D.
中科院分区：
文献类型：
--
作者：
Cui, Y.;Lv, S.;Yang, D.
全氟辛烷磺酸(PFOS)是一种普遍存在的污染物，已被广泛应用于各种工业领域。目前，很少有研究证明长期接触全氟辛烷磺酸对脂类代谢的影响，特别是在水生生物中。本研究明确了长期暴露于低水平全氟辛烷磺酸对F0成年斑马鱼及其F1后代脂代谢的影响。我们的发现显示，0.5μM全氟辛烷磺酸处理的F0雄鼠肝脏出现严重的脂肪变性，与肝脏和肠道的物质运输或代谢有关的超微结构发生了显著的变化(线粒体和内质网异常，细胞内小管内微绒毛排列紊乱)。为了研究全氟辛烷磺酸暴露的潜在跨代效应，采用实时定量聚合酶链式反应技术检测了长期暴露的亲鱼F1中与脂代谢相关的早期基因表达。结果表明，F1的Leptinα、Kiss1、Xdh和InsR显著上调，而DGAT1b、Hb9和ApoA1下调。这些发现提供了全氟辛烷磺酸慢性暴露对F0和F1的脂质代谢产生不利影响的证据，并证明了使用斑马鱼作为全氟辛烷磺酸慢性毒性筛选的替代模型的有效性。
Perfluorooctanesulfonic acid (PFOS), a ubiquitous contaminant, has been used in various industrial applications. Currently few studies have documented the effects of chronic PFOS exposure on lipid metabolism, especially in aquatic organisms. The present study defined the effects of chronic exposure to low level of PFOS on lipid metabolism in F0 adult zebrafish and F1 offspring. Our findings revealed a severe fatty degeneration in the liver of F0 males treated with 0.5 μM PFOS and significant ultrastructure changes associated with substance transport or metabolism in liver and intestines (abnormal mitochondria and endoplasmic reticulum, disordered arrangement of inner microvilli within intracellular canaliculus). To address the potential trans-generational effects of PFOS exposure, the early gene expression related to lipid metabolism was measured by real-time quantitative polymerase chain reaction in F1 derived from chronically exposed parental fish. The results indicated that lepa (leptin α), kiss1 (kisspeptins), xdh (xanthine dehydrogenases), and insr (insulin receptor) were significantly upregulated in F1 while dgat1b (diacylglycerol O-acyltransferase), hb9 (motor neuron/pancreas homeobox), and Apoa1 (apolipoprotein A-I) were downregulated. These findings provided evidence that PFOS chronic exposure adversely impacts lipid metabolism in both F0 and F1 and demonstrated the validity of using zebrafish as an alternative model for PFOS chronic toxicity screening.