Neuron numbers in the sensory trigeminal nuclei of the rat:: A GABA- and glycine-immunocytochemical and stereological analysis
Neuron numbers in the sensory trigeminal nuclei of the rat:: A GABA- and glycine-immunocytochemical and stereological analysis
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DOI：
10.1002/cne.20778
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发表时间：
2005-12-26
影响因子：
2.5
通讯作者：
Lagares, A
中科院分区：
文献类型：
--
作者：
Avendaño, C;Machín, R;Lagares, A
用体视学方法测定了成年大鼠三叉神经感觉核中表达GABA和甘氨酸的神经元的体积、神经元总数和数量。主核（Vp）的平均体积为1.38 +/- 0.13 mm(3)（平均+/- SD），口萼（Vo）的平均体积为1.59 +/- 0.06 mm，内插（Vip）的平均体积为2.63 +/- 0.34 mm, 3.73 +/- 0 mm。11为n尾肌。神经元总数为31900 +/- 2200 (Vp), 21100 +/- 3300 (Vo), 61600 +/- 8300 （Vip）和159100 +/- 25300 （Vc）。免疫反应性（-ir）神经元被分类为强染色或弱染色，这取决于定性标准，通过密度分析交叉检查。GABA-ir细胞在Vc中最丰富，在细胞核内呈背向梯度增加。Vip和Vp密度较低。强标记GABA-ir神经元的平均总数在Vp组的1800个到Vip组的7800个和Vc组的22900个之间，在受试者之间差异显著。甘氨酸-ir神经元在所有核中数量更多，密度更均匀。所有细胞核中均以强标记Gly-ir细胞为主，其总数从Vp的9400个到Vip的24300个和Vc的34200个不等。所有细胞核中有相当一部分免疫标记的神经元共表达GABA和甘氨酸。总的来说，所有对GABA免疫反应强烈的神经元都很小，而共表达Gly的弱GABA-ir细胞则较大。在Vc中，三分之一的神经元具有免疫反应性，其中16.6%为GABA单标记，31.6%为甘氨酸单标记。其余51.8%表达不同组合的GABA和甘氨酸，具有强双重标记的占22.6%。
The volume, total neuron number, and number of GABA- and glycine-expressing neurons in the sensory trigeminal nuclei of the adult rat were estimated by stereological methods. The mean volume is 1.38 +/- 0.13 mm(3) (mean +/- SD) for the principal nucleus (Vp), 1.59 +/- 0.06 for the n. oralis (Vo), 2.63 +/- 0.34 for the n. interpolaris (Vip), and 3.73 +/- 0. 11 for the n. caudalis NO. The total neuron numbers are 31,900 +/- 2,200 (Vp), 21,100 +/- 3,300 (Vo), 61,600 +/- 8,300 (Vip), and 159,100 +/- 25,300 (Vc). Immunoreactive (-ir) neurons were classified as strongly stained or weakly stained, depending on qualitative criteria, cross-checked by a densitometric analysis. GABA-ir cells are most abundant in Vc, in an increasing rostrocaudal gradient within the nucleus. Lower densities are found in Vip and Vp. The mean total number of strongly labeled GABA-ir neurons ranges between 1,800 in Vp to 7,800 in Vip and 22,900 in Vc, and varies notably between subjects. Glycine-ir neurons are more numerous and display more homogeneous densities in all nuclei. Strongly labeled Gly-ir cells predominate in all nuclei, their total number ranging between 9,400 in Vp to 24,300 in Vip and 34,200 in Vc. A substantial fraction of immunolabeled neurons in all nuclei coexpress GABA and glycine. In general, all neurons strongly immunoreactive for GABA are small, while weakly GABA-ir cells which coexpress Gly are larger. In Vc, one-third of all neurons are immunoreactive: 16.6% of them are single-labeled for GABA and 31.6% are single-labeled for glycine. The remaining 51.8% express GABA and glycine in different combinations, with those showing strong double labeling accounting for 22.6%.