Antitumor effect of acridine orange under ultrasonic irradiation in vitro.
Antitumor effect of acridine orange under ultrasonic irradiation in vitro.
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发表时间：
2007-11
影响因子：
2
通讯作者：
Norio Suzuki;K. Okada;Shuichi Chida;Chiyo Komori;Y. Shimada;Toshio Suzuki
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作者：
Norio Suzuki;K. Okada;Shuichi Chida;Chiyo Komori;Y. Shimada;Toshio Suzuki
背景声动力协同抗肿瘤治疗是一种很有前途的肿瘤治疗新方法。材料与方法为确定吖啶橙子（AO）存在下的超声（US）的抗肿瘤作用，在体外测试1.0 μ g/ml AO溶液作为声动力化合物对肉瘤180细胞的抗肿瘤作用。结果2.0W/cm ~ 2超声照射60 s后，AO存在时肿瘤细胞存活率明显低于无AO存在时（P < 0.001）。在AO处理组中，肿瘤细胞大多是碎片。当D-甘露醇或L-组氨酸沿着AO时，照射后肿瘤细胞的存活率明显高于单独AO。结论AO在超声辐照下具有抗肿瘤活性，其对肿瘤细胞的损伤作用与单线态氧和羟基自由基的产生有关。
BACKGROUND Sonodynamic synergistic antitumor therapy is a promising new methodology for cancer treatment. MATERIALS AND METHODS To determine the antitumor effects of ultrasound (US) in the presence of acridine orange (AO), 1.0 microg/ml solution of AO was tested as a sonodynamic compound against sarcoma 180 cells in vitro. RESULTS After US irradiation at 2.0 W/cm2 for 60 sec, the survival rate of tumor cells in the presence of AO was significantly lower than in its absence (p < 0.001). In the AO-treated group, the tumor cells were mostly fragmented. When D-mannitol or L-histidine was used along with the AO, the survival rate of tumor cells after irradiation was significantly higher than that when AO alone was applied. CONCLUSION AO could exhibit useful antitumor activity under US irradiation, and the generation of singlet oxygen and hydroxyl radicals is involved in the processes of tumor cell damage.