Long non-coding RNA FOXF1 adjacent non-coding developmental regulatory RNA inhibits growth and chemotherapy resistance in non-small cell lung cancer
Long non-coding RNA FOXF1 adjacent non-coding developmental regulatory RNA inhibits growth and chemotherapy resistance in non-small cell lung cancer
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DOI：
10.5114/aoms.2019.86707
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发表时间：
2019-10-01
影响因子：
3.8
通讯作者：
Han, Yun
中科院分区：
文献类型：
--
作者：
Xu, Ran;Han, Yun
前言：肺癌是地球仪最常见的恶性肿瘤之一。最常见的类型是非小细胞肺癌（NSCLC）。FOXF 1邻近非编码发育调节RNA（FENDRR）基因是一种lncRNA，在非小细胞肺癌（NSCLC）中低表达，具有抑癌作用。MTT法检测细胞增殖及化疗耐药性。结果：FENDRR在非小细胞肺癌组织和细胞中的表达较对照组低，FENDRR的低表达与非小细胞肺癌的TNM分期高、分化差有关，可能是一个有希望的预后指标。FENDRR增强可抑制A549细胞的增殖能力，促进细胞凋亡。FENDRR在顺铂不敏感的NSCLC组织和细胞中的表达明显低于顺铂敏感的NSCLC组织和细胞。FENDRR增强后A549/DDP细胞对顺铂的耐药性降低，对顺铂的IC 50值明显降低。FENDRR的上调抑制了5 μ g/ml DDP处理的A549/DDP细胞的细胞活力。TCGA Pan-Cancer（PANCAN）结果显示FENDRR在肺癌中的表达与ABCC 10的表达呈负相关，Western blot结果显示FENDRR上调可抑制A549/DDP细胞中ABCC 10的表达。
Introduction: Lung cancer is one of the most common malignant neoplasms around the globe. Its most common type is non-small cell lung cancer (NSCLC). The FOXF1 adjacent non-coding developmental regulatory RNA (FENDRR) gene is an lncRNA which has been reported to show low expression and a tumor suppressor role in NSCLC.Material and methods: The expression of FENDRR in NSCLC patients' tissues and cell line was detected by quantitative real-time PCR. MTT assay was used to detect cell proliferation and chemotherapy resistance. Cell apoptosis was measured by flow cytometry.Results: The expression of FENDRR was low in NSCLC tissues and cells in contrast to control tissues and cells, and low FENDRR expression correlated with high TNM stages and poor differentiation of NSCLC, and could be a promising prognostic factor for NSCLC. FENDRR enhancement could inhibit the proliferation ability and advance cell apoptosis of A549 cells. The expression of FENDRR in NSCLC tissues and cells insensitive to cisplatin was much lower than that in NSCLC tissues and cells sensitive to cisplatin. The chemotherapy resistance to cisplatin of A549/DDP cells was depressed by FENDRR enhancement, and IC50 for cisplatin presented a conspicuous depression. FENDRR up-regulation inhibited cell viability of A549/DDP cells under treatment with 5 mu g/ml DDP. TCGA Pan-Cancer (PANCAN) showed that the expression of FENDRR was negatively correlated with the expression of ABCC10 in lung cancer, and our western blot found that FENDRR up-regulation inhibited the expression of ABCC10 in A549/DDP cells.Conclusions: LncRNA FENDRR has low expression in NSCLC and functions as a potential tumor-suppressing gene to inhibit growth and chemotherapy resistance of NSCLC cells.