Strand scission of deoxyribonucleic acid by neocarzinostatin, auromomycin, and bleomycin: studies on base release and nucleotide sequence specificity.
Strand scission of deoxyribonucleic acid by neocarzinostatin, auromomycin, and bleomycin: studies on base release and nucleotide sequence specificity.
复制标题
新制癌菌素、金霉素和博来霉素对脱氧核糖核酸的链断裂：碱基释放和核苷酸序列特异性的研究。
DOI：
10.1021/bi00529a039
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Goldberg,IH
中科院分区：
文献类型：
--
作者：
Takeshita,M;Kappen,LS;Grollman,AP;Eisenberg,M;Goldberg,IH
材料和方法材料。限制性内切酶HaeIII、Taql、Hhal和末端脱氧核苷酸转移酶购自Bethesda Research Laboratories，细菌碱性磷酸酶购自Worthington Biochemical，多核苷酸激酶购自PL Biochemicals，[γ-32 P] ATP（比活度~ 3000 Ci/mmol）和[α-32 P] ATP（比活度~ 2000 Ci/mmol）购自ICN。博来霉素A2（Blm A2）（批次71 L 489）和他来霉素B（Tim）（批次35 F-1-21）的无铜样品由布里斯托实验室（锡拉丘兹，纽约州）提供;博来霉素B6（Blm B6）、腐草霉素G（Phlm G）和腐草霉素Pep（Phlm-Pep）由G. W. Grigg，Sydney，Australia;培洛霉素（Blm-Pep），NSC-276382，获自National Cancer Institute，Bethesda，MD。博来霉素BL（Blm Bl '）是H. Umezawa; NCS由W. T.布里斯托实验室的布拉德纳，AUR从T. S. A. Sarny通过教授H的礼貌。梅泽非
Materials and Methods Materials. Restriction enzymes Haelll, Taql, Hhal, and terminal deoxynucleotide transferase were purchased from Bethesda Research Laboratories, bacterialalkaline phospha-tase was from Worthington Biochemical, polynucleotidekinase was from PL Biochemicals, and [y-32P] ATP (specific activity~ 3000 Ci/mmol) and [a-32P] ATP (specific activity~ 2000 Ci/mmol) were from ICN. Copper-free samples of bleomycin A2 (Blm A2)(lot 71L 489) and tallysomycin B (Tim),(lot 35F-1-21) were provided by Bristol Laboratories, Syracuse, NY; bleomycin B6 (Blm B6), phleomycin G (Phlm G) and phleomycin Pep (Phlm-Pep) were gifts from G. W. Grigg, Sydney, Australia; pepleomycin (Blm-Pep), NSC-276382, was obtained from the National Cancer Institute, Bethesda, Md. Bleomycin BL (Blm Bl') was a gift from Professor H. Umezawa; NCS was provided by Dr. W. T. Bradner of Bristol Laboratories, and AUR was obtained from Dr. T. S. A. Sarny through the courtesy of Professor H. Umezawa. The non-