PRODUCTION OF ANTISERUM TO [D-ALA2]DELTORPHIN I AND ITS IMMUNOHISTOCHEMICAL APPLICATION TO THE MOUSE-BRAIN
PRODUCTION OF ANTISERUM TO [D-ALA2]DELTORPHIN I AND ITS IMMUNOHISTOCHEMICAL APPLICATION TO THE MOUSE-BRAIN
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DOI：
10.1097/00001756-199208000-00003
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发表时间：
1992-08-01
期刊：
影响因子：
1.7
通讯作者：
KIMURA, H
中科院分区：
文献类型：
--
作者：
ABE, H;TOOYAMA, I;KIMURA, H
半抗原[D-Ala 2]deltorphin I（DADTI：Tyr-D-Ala-Phe-Asp-Val-Val-Gly-NH 2）是δ-阿片受体的一种高选择性配体，在家兔中制备了抗血清。用免疫斑点法测定，抗血清识别62.5pmol DADTI，但不与4 nmol免疫原载体蛋白反应。虽然抗血清与异构体[L-Ala 2]deltorphin I反应相同，但实际上与其他类似物如[D-Ala 2]deltorphin II（Tyr-D-Ala-Phe-Glu-Val-Val-Gly-NH 2）和缺乏C-末端甘氨酰胺的类似肽没有发生交叉反应。因此，免疫识别的主要表位似乎在C-末端区域，已知该区域是δ-阿片受体选择性的特异性结构域。免疫组织化学研究表明，在小鼠脑的某些特定系统的阳性神经元结构。
ANTISERUM to haptenic [D-Ala2]deltorphin I (DADTI: Tyr-D-Ala-Phe-Asp-Val-Val-Gly-NH2), a highly selective ligand for delta-opioid receptors, was produced in rabbits. By immunospot assay, the antiserum recognized 62.5 pmol DADTI but failed to react even with 4 nmol carrier protein of the immunogen. Although the antiserum reacted equally with an isomer [L-Ala2]deltorphin I, virtually no cross-reaction occurred with other analogues such as [D-Ala2]deltorphin II (Tyr-D-Ala-Phe-Glu-Val-Val-Gly-NH2) and similar peptides lacking the C-terminal glycine amide. Therefore, the major epitope for immunorecognition appeared to be in the C-terminal region which is known to be the specific domain for delta-opioid receptor selectivity. Immunohistochemical study using this antiserum revealed positive neuronal structures in some specific systems of the mouse brain.