The transmembrane protein TMEM182 promotes fat deposition and alters metabolomics and lipidomics
The transmembrane protein TMEM182 promotes fat deposition and alters metabolomics and lipidomics
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DOI：
10.1016/j.ijbiomac.2023.129144
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发表时间：
2024-01-05
影响因子：
8.2
通讯作者：
Luo，Wen
中科院分区：
文献类型：
--
作者：
Chen，Genghua;Lin，Zetong;Luo，Wen
TMEM182是一种在肌肉和脂肪组织中高度表达的跨膜蛋白，在肌肉细胞分化、代谢和信号传导中起着至关重要的作用。然而，其在脂肪沉积和代谢中的作用尚不清楚。在这项研究中，我们使用过表达和敲除模型来检测tmem182对脂肪合成和代谢的影响。我们的研究结果表明，attmem182过表达增加了脂肪合成相关基因的表达，促进了前脂肪细胞向脂肪细胞的分化。敲除intmem182小鼠，腹部脂肪沉积显著减少。RNA测序结果显示，attmem182在前脂肪细胞中的过表达增强了脂肪形成、ecm受体相互作用和细胞粘附相关通路的活性。此外，我们的UPLC-MS/MS分析表明，attmem182显著改变了鸡胸肌的代谢物和脂质含量和组成。具体来说，tmem182增加了鸡胸肌中氨基酸及其衍生物的含量，促进了氨基酸代谢途径。脂质组学还发现，tmem182过表达后，胸肌中甘油磷脂、鞘磷脂和磷脂的含量显著增加。这些发现表明，TMEM182在调节脂肪沉积和代谢中起着至关重要的作用，使其成为治疗肥胖相关疾病和动物育种的潜在靶点。
TMEM182, a transmembrane protein highly expressed in muscle and adipose tissues, plays a crucial role in muscle cell differentiation, metabolism, and signaling. However, its role in fat deposition and metabolism is still unknown. In this study, we used overexpression and knockout models to examine the impact ofTMEM182on fat synthesis and metabolism. Our results showed thatTMEM182overexpression increased the expression of fat synthesis-related genes and promoted the differentiation of preadipocytes into fat cells. InTMEM182knockout mice, there was a significant decrease in abdominal fat deposition. RNA sequencing results showed thatTMEM182overexpression in preadipocytes enhanced the activity of pathways related to fat formation, ECM-receptor interaction, and cell adhesion. Furthermore, our analysis using UPLC-MS/MS showed thatTMEM182significantly altered the metabolite and lipid content and composition in chicken breast muscle. Specifically,TMEM182increased the content of amino acids and their derivatives in chicken breast muscle, promoting amino acid metabolic pathways. Lipidomics also revealed a significant increase in the content of glycerophospholipids, sphingolipids, and phospholipids in the breast muscle afterTMEM182overexpression. These findings suggest that TMEM182 plays a crucial role in regulating fat deposition and metabolism, making it a potential target for treating obesity-related diseases and animal breeding.