Second-harmonic generation imaging of collagen in ancient bone.
Second-harmonic generation imaging of collagen in ancient bone.
复制标题
古骨中胶原蛋白的二次谐波生成成像。
DOI：
10.1016/j.bonr.2017.10.005
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发表时间：
2017
期刊：
影响因子：
2.5
通讯作者：
Taylor,S
中科院分区：
文献类型：
--
作者：
Thomas,B;McIntosh,D;Fildes,T;Smith,L;Hargrave,F;Islam,M;Thompson,T;Layfield,R;Scott,D;Shaw,B;Burrell,CL;Gonzalez,S;Taylor,S
二次谐波生成成像 (SHG) 捕获组织表面附近的三螺旋胶原蛋白分子。生物医学研究通常利用各种成像软件包来量化 SHG 信号，以了解现代组织样本（包括骨骼）中胶原蛋白的含量和分布估计。首次使用 SHG 对现代、中世纪和冰河时代的骨骼样本进行成像，以测试 SHG 对不同时代、环境和分类群的古代骨骼的适用性。包括拉曼光谱、FTIR 光谱、放射性碳测年方案和基于质谱的蛋白质测序在内的四种独立技术证实了蛋白质的存在，这与 SHG 成像检测古代骨胶原的假设一致。这些结果表明，未来的研究有可能利用二次谐波成像来提供对古代骨骼组成的新见解，表征古代骨骼疾病，研究不同类群内部和之间的胶原蛋白保存，并监测不同沉积环境中胶原蛋白的腐烂情况。
Second-harmonic generation imaging (SHG) captures triple helical collagen molecules near tissue surfaces. Biomedical research routinely utilizes various imaging software packages to quantify SHG signals for collagen content and distribution estimates in modern tissue samples including bone. For the first time using SHG, samples of modern, medieval, and ice age bones were imaged to test the applicability of SHG to ancient bone from a variety of ages, settings, and taxa. Four independent techniques including Raman spectroscopy, FTIR spectroscopy, radiocarbon dating protocols, and mass spectrometry-based protein sequencing, confirm the presence of protein, consistent with the hypothesis that SHG imaging detects ancient bone collagen. These results suggest that future studies have the potential to use SHG imaging to provide new insights into the composition of ancient bone, to characterize ancient bone disorders, to investigate collagen preservation within and between various taxa, and to monitor collagen decay regimes in different depositional environments.